Biomaterial Database

Immunological analysis of Opisthorchis and Clonorchis antigens. 1990

S Sirisinha, and D Sahassananda, and D Bunnag, and H J Rim

Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.

Immunoreactive components of Opisthorchis viverrini and Clonorchis sinensis were analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted between Opisthorchis and Clonorchis. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product of O. viverrini reacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific for O. viverrini.

UI	MeSH Term	Description	Entries
D009889	Opisthorchiasis	Infection with flukes of the genus Opisthorchis.	Opisthorchis Infection,Opisthorchis felineus Infection,Opisthorchis viverrini Infection,Infection, Opisthorchis,Infection, Opisthorchis felineus,Infection, Opisthorchis viverrini,Infections, Opisthorchis,Infections, Opisthorchis felineus,Infections, Opisthorchis viverrini,Opisthorchiases,Opisthorchis Infections,Opisthorchis felineus Infections,Opisthorchis viverrini Infections
D009891	Opisthorchis	A genus of trematode liver flukes of the family Opisthorchidae. It consists of the following species: O. felineus, O. noverca (Amphimerus noverca), and O. viverrini. The intermediate hosts are snails, fish, and AMPHIBIANS.	Opisthorchis felineus,Opisthorchis viverrini,Opisthorchi,Opisthorchis viverrinus,viverrini, Opisthorchis
D003003	Clonorchiasis	Infection of the biliary passages with CLONORCHIS SINENSIS, also called Opisthorchis sinensis. It may lead to inflammation of the biliary tract, proliferation of biliary epithelium, progressive portal fibrosis, and sometimes bile duct carcinoma. Extension to the liver may lead to fatty changes and cirrhosis. (From Dorland, 27th ed)	Clonorchis Infection,Clonorchis sinensis Infection,Opisthorchis sinensis Infection,Clonorchiases,Clonorchis Infections,Clonorchis sinensis Infections,Infection, Clonorchis,Infection, Clonorchis sinensis,Infection, Opisthorchis sinensis,Opisthorchis sinensis Infections
D003004	Clonorchis sinensis	A species of trematode flukes of the family Opisthorchidae. Many authorities consider this genus belonging to Opisthorchis. It is common in China and other Asiatic countries. Snails and fish are the intermediate hosts.	Opisthorchis sinensis,Clonorchis sinenses,Opisthorchis sinenses,sinenses, Opisthorchis,sinensis, Clonorchis
D003429	Cross Reactions	Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen.	Cross Reaction,Reaction, Cross,Reactions, Cross
D004591	Electrophoresis, Polyacrylamide Gel	Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.	Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004797	Enzyme-Linked Immunosorbent Assay	An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.	ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000909	Antibodies, Helminth	Immunoglobulins produced in a response to HELMINTH ANTIGENS.	Helminth Antibodies