Magnetic beads-based electrochemical immunosensor for detection of pseudorabies virus antibody in swine serum. 2011

Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
School of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, China.

A novel magnetic electrochemical immunosensor has been developed for the detection of pseudorabies virus antibody in swine serum. The magnetic glass carbon electrode was fabricated to manipulate magnetic beads for the direct sensing applications. Magnetic beads were employed as the platforms for the immobilization and immunoreaction process, and gold nanoparticles were chosen as electroactive labels for the electrochemical detection. The parameters concerning the assay strategy were carefully investigated. Under the optimal conditions, the linear response range of pseudorabies virus antibody dilution ratio (standard positive serum) was 1:250 to 1:1000 with a detection limit of 1:1000. Finally, this developed immunoassay method was successfully applied in the detection of pseudorabies virus antibody in swine serum, and had a good diagnostic accordance in comparison with ELISA.

UI MeSH Term Description Entries
D007118 Immunoassay A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance. Immunochromatographic Assay,Assay, Immunochromatographic,Assays, Immunochromatographic,Immunoassays,Immunochromatographic Assays
D011557 Pseudorabies A highly contagious herpesvirus infection affecting the central nervous system of swine, cattle, dogs, cats, rats, and other animals. Aujeszky's Disease,Aujeszky Disease,Aujeszkys Disease
D011558 Herpesvirus 1, Suid A species of VARICELLOVIRUS producing a respiratory infection (PSEUDORABIES) in swine, its natural host. It also produces an usually fatal ENCEPHALOMYELITIS in cattle, sheep, dogs, cats, foxes, and mink. Aujeszky's Disease Virus,Swine Herpesvirus 1,Aujeszky Disease Virus,Herpesvirus 1 (alpha), Suid,Herpesvirus Suis,Pseudorabies Virus,Suid Herpesvirus 1,Aujeszkys Disease Virus,Herpesvirus 1, Swine,Pseudorabies Viruses,Virus, Pseudorabies,Viruses, Pseudorabies
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D055664 Electrochemical Techniques The utilization of an electrical current to measure, analyze, or alter chemicals or chemical reactions in solution, cells, or tissues. Electrochemical Technics,Electrochemical Technic,Electrochemical Technique,Technic, Electrochemical,Technics, Electrochemical,Technique, Electrochemical,Techniques, Electrochemical
D057230 Limit of Detection Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure. Limits of Detection,Detection Limit,Detection Limits
D059346 Magnets Objects that produce a magnetic field. Electromagnets,Ferrimagnet,Ferrimagnets,Ferromagnet,Ferromagnets,Electromagnet,Magnet

Related Publications

Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
May 2014, Journal of nanoscience and nanotechnology,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
September 2015, Analytical biochemistry,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
July 2020, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
March 2022, Analytical and bioanalytical chemistry,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
November 2015, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
December 1978, American journal of veterinary research,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
March 1980, Journal of clinical microbiology,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
August 2012, Chemistry, an Asian journal,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
March 2012, Talanta,
Fang Li, and Rui Zhou, and Kaihong Zhao, and Huanchun Chen, and Yonggang Hu
May 2019, The Analyst,
Copied contents to your clipboard!