Characterization of a monoclonal anti-capsid antibody that cross-reacts with three major primate lentivirus lineages. 2012

Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
BIOQUAL, Inc., Rockville, MD 20850, USA. bsanders@bioqual.com

Mouse monoclonal antibodies with varying specificities against the Gag capsid of simian and human immunodeficiency virus (SIV/HIV) were generated by immunizing mice with whole inactivated SIVagmTYO-1. Monoclonal antibody AG3.0 showed the broadest reactivity recognizing the Gag capsid protein (p24-27) and Gag precursors p38, p55, and p150 of HIV-1, HIV-2, SIVmac, and SIVagm. Using overlapping peptides, the AG3.0 epitope was mapped in capsid to a sequence (SPRTLNA) conserved among HIV-1, HIV-2, SIVrcm, SIVsm/mac, and SIVagm related viruses. Because of its broad cross-reactivity, AG3.0 was used to develop an antigen capture assay with a lower detection limit of 100 pg/ml HIV-1 Gag p24. Interestingly, AG3.0 was found to have a faster binding on/off rate for SIVagmVer and SIVmac Gag than for SIVagmSab Gag, possibly due to differences outside the SPRTLNA motif. In addition, the ribonucleic acid (RNA) coding for AG3.0 was sequenced to facilitate the development of humanized monoclonal antibodies.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003429 Cross Reactions Serological reactions in which an antiserum against one antigen reacts with a non-identical but closely related antigen. Cross Reaction,Reaction, Cross,Reactions, Cross
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000911 Antibodies, Monoclonal Antibodies produced by a single clone of cells. Monoclonal Antibodies,Monoclonal Antibody,Antibody, Monoclonal
D015302 Simian Immunodeficiency Virus Species of the genus LENTIVIRUS, subgenus primate immunodeficiency viruses (IMMUNODEFICIENCY VIRUSES, PRIMATE), that induces acquired immunodeficiency syndrome in monkeys and apes (SAIDS). The genetic organization of SIV is virtually identical to HIV. SIV (Simian immunodeficiency virus),Immunodeficiency Viruses, Simian,Simian Immunodeficiency Viruses,Immunodeficiency Virus, Simian
D015496 CD4-Positive T-Lymphocytes A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes. T4 Cells,T4 Lymphocytes,CD4-Positive Lymphocytes,CD4 Positive T Lymphocytes,CD4-Positive Lymphocyte,CD4-Positive T-Lymphocyte,Lymphocyte, CD4-Positive,Lymphocytes, CD4-Positive,T-Lymphocyte, CD4-Positive,T-Lymphocytes, CD4-Positive,T4 Cell,T4 Lymphocyte

Related Publications

Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
January 2016, mAbs,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
August 1983, FEBS letters,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
August 2006, Food additives and contaminants,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
January 1985, Hybridoma,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
January 1985, Human immunology,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
May 1987, Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
April 1982, Science (New York, N.Y.),
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
June 1987, Histopathology,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
August 1996, Hybridoma,
Brigitte E Sanders-Beer, and Magdalena Eschricht, and Janna Seifried, and Vanessa M Hirsch, and Jonathan S Allan, and Stephen Norley
June 1991, The British journal of dermatology,
Copied contents to your clipboard!