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Mn2(+)-activated aspartate aminopeptidase activity, subcellular localization in young and adult rat brain. 1990
Department of Physiology, Faculty of Medicine, University of the Basque Country, Leioa, Vizcaya, Spain.
The levels of soluble and membrane-bound aminopeptidase activities were assayed in subcellular fractions from young (4 weeks old) and adult (20 weeks old) rat brains, using Asp-2-naphthylamide as substrate. The young rats showed the highest soluble and membrane-bound levels of activity in the microsomal fraction but no differences among fractions were found at 20 weeks of age. The membrane-bound activity was significantly higher than the soluble one in all subcellular fractions of young rats. Soluble activity of the homogenate and the mitochondrial fraction was significantly increased in adult animals when compared to that of younger ones.
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