BIOMAT Database Logo BIOMAT Database Logo

Arsenate toxicity and stress responses in the freshwater ciliate Tetrahymena pyriformis. 2012

Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.

The arsenic metabolism in different biological organisms has been studied extensively. However, little is known about protozoa. Herein, we investigated the cell stress responses of the freshwater ciliate Tetrahymena pyriformis to arsenate toxicity. An acute toxicity assay revealed an 18-h EC(50) arsenate concentration of ca. 40 μM, which caused significant changes in the cell shape, growth and organism mobility. Whereas, under exposure to 30 μM arsenate, T. pyriformis could grow reasonably well, indicating a certain resistance of this organism. Arsenic speciation analysis revealed that 94-98% of the total arsenate in cells of T. pyriformis could be transformed to monomethylarsonic acid, dimethylarsinic acid and a small proportion of arsenite after 18 h of arsenate exposure, thus indicating the major detoxification pathway by arsenic oxidation/reduction and biomethylation. Finally, comparative proteomic analysis unveiled significant changes in the expression of multiple proteins involved in anti-oxidation, sugar and energy metabolism, proteolysis, and signal transduction. Our results revealed multiple pathways of arsenate detoxification in T. pyriformis, and indicated that protozoa may play important roles in the biogeochemical cycles of arsenic.

UI MeSH Term Description Entries
D008124 Locomotion Movement or the ability to move from one place or another. It can refer to humans, vertebrate or invertebrate animals, and microorganisms. Locomotor Activity,Activities, Locomotor,Activity, Locomotor,Locomotor Activities
D002470 Cell Survival The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability. Cell Viability,Cell Viabilities,Survival, Cell,Viabilities, Cell,Viability, Cell
D005618 Fresh Water Water containing no significant amounts of salts, such as water from RIVERS and LAKES. Freshwater,Fresh Waters,Freshwaters,Water, Fresh,Waters, Fresh
D001149 Arsenates Inorganic or organic salts and esters of arsenic acid.
D001711 Biotransformation The chemical alteration of an exogenous substance by or in a biological system. The alteration may inactivate the compound or it may result in the production of an active metabolite of an inactive parent compound. The alterations may be divided into METABOLIC DETOXICATION, PHASE I and METABOLIC DETOXICATION, PHASE II.
D013312 Stress, Physiological The unfavorable effect of environmental factors (stressors) on the physiological functions of an organism. Prolonged unresolved physiological stress can affect HOMEOSTASIS of the organism, and may lead to damaging or pathological conditions. Biotic Stress,Metabolic Stress,Physiological Stress,Abiotic Stress,Abiotic Stress Reaction,Abiotic Stress Response,Biological Stress,Metabolic Stress Response,Physiological Stress Reaction,Physiological Stress Reactivity,Physiological Stress Response,Abiotic Stress Reactions,Abiotic Stress Responses,Abiotic Stresses,Biological Stresses,Biotic Stresses,Metabolic Stress Responses,Metabolic Stresses,Physiological Stress Reactions,Physiological Stress Responses,Physiological Stresses,Reaction, Abiotic Stress,Reactions, Abiotic Stress,Response, Abiotic Stress,Response, Metabolic Stress,Stress Reaction, Physiological,Stress Response, Metabolic,Stress Response, Physiological,Stress, Abiotic,Stress, Biological,Stress, Biotic,Stress, Metabolic
D013769 Tetrahymena pyriformis A species of ciliate protozoa used extensively in genetic research. Tetrahymena pyriformi,pyriformi, Tetrahymena
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan
D020543 Proteome The protein complement of an organism coded for by its genome. Proteomes

Related Publications

Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Deoxyribonucleoproteins from the ciliate Tetrahymena pyriformis GL.
June 1967, Proceedings of the National Academy of Sciences of the United States of America,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Non-specific activation of mouse peritoneal macrophages by a freshwater ciliate, Tetrahymena pyriformis.
June 2000, The Korean journal of parasitology,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Quantitative structure-activity study of the toxicity of benzonitriles to the ciliate Tetrahymena pyriformis.
January 1995, SAR and QSAR in environmental research,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
pH-stress and toxicity of nitrophenols to Tetrahymena pyriformis.
November 2003, Bulletin of environmental contamination and toxicology,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
The macronuclear polyubiquitin gene of the ciliate Tetrahymena pyriformis.
January 1991, DNA sequence : the journal of DNA sequencing and mapping,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Isolation of macronuclei from the ciliate Tetrahymena pyriformis GL.
December 1965, Nature,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Ca2+ transport in mitochondria of the ciliate protozoan Tetrahymena pyriformis.
February 1984, Cell calcium,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Griseofulvin: generation time and ATP changes in the ciliate Tetrahymena pyriformis.
January 1995, Life sciences,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Polyterpenoids as cholesterol and tetrahymanol surrogates in the ciliate Tetrahymena pyriformis.
May 1988, Biochimica et biophysica acta,
Yong-Yu Zhang, and Jun Yang, and Xi-Xiang Yin, and Su-Ping Yang, and Yong-Guan Zhu
Immunological responses of the toad, Xenopus laevis, to the antigens of the ciliate, Tetrahymena pyriformis.
September 1968, Immunology,
Copied contents to your clipboard!