1. Fat deposition in abdominal, mesenterial, sartorial and gizzard adipose tissues (AT), liver, breast muscle, skin and carcase was studied in male broilers, selected for high (HF) and low (LF) abdominal fat and fed on diets differing in energy density and total fat content. 2. There were no significant differences in body weight in the experimental groups. The relative weight (g/kg body weight) of the dissected adipose tissues was higher in HF than in LF birds. Fat concentration in the AT (sartorial excepted), skin and body was higher in the HF compared with the LF birds. The lines did not differ significantly in liver and breast muscle fat content. 3. Abdominal AT was affected by selection or dietary fat more than other AT and total body fat. 4. In the HF birds increasing energy density from 12.3 to 13.4 MJ/kg (dietary fat kept constant: 5.46 g/MJ) significantly increased the weight of the abdominal, mesenterial and sartorial AT. Increasing dietary fat (at both energy densities) decreased the weight of the AT, whereas increasing both energy and fat did not affect it. In the LF birds, similar but milder and insignificant trends were observed. It is suggested that this interaction has biological significance.