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Effects of an aqueous extract of orthosiphon thymiflorus on acetyl choline induces contraction on isolated frog rectus abdominis muscle were studies. The aqueous extract of orthosiphon thymiflorus produced significant inhibitory effect on the dose response curve of acetyl choline. It is concluded that the aqueous extract of orthosiphon thymiflorus may exert an inhibitory effect on the skeletal muscle contraction and may be due to inhibition of the effect of acetylcholine at the receptor site.
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