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Spectrophotometric determination of acetone using vanillin. 1990

S Amlathe, and V K Gupta
Department of Chemistry, Ravishankar University, Raipur, India.

A sensitive method is described for the spectrophotometric determination of acetone in water. Acetone reacts with vanillin in alkaline medium forming a yellow-orange dye with an absorption maximum at 430 nm. Beer's law is obeyed in the range from 0.5 to 5 p.p.m. of acetone in water. Molar absorptivity and Sandell's sensitivity were found to be 11.0 x 10(3) +/- 100 l mol-1 cm-1 and 0.005 micrograms cm-2, respectively. The important parameters for complete colour development were optimised and the method was used for the determination of acetone in biological samples. The method was also applied successfully to the determination of acetone in air after absorption in sodium hydrogen sulphite solution.

UI MeSH Term Description Entries
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000096 Acetone A colorless liquid used as a solvent and an antiseptic. It is one of the ketone bodies produced during ketoacidosis.
D000388 Air The mixture of gases present in the earth's atmosphere consisting of oxygen, nitrogen, carbon dioxide, and small amounts of other gases.
D001547 Benzaldehydes Compounds that consist of a benzene ring with a formyl group. They occur naturally in the seeds of many fruits and are used as FLAVORING AGENTS and fragrances, as well as precursors in pharmaceutical and plastics manufacturing.
D013056 Spectrophotometry, Ultraviolet Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Ultraviolet Spectrophotometry

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