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An electrophoretic method for separating well-defined large DNA fragments from higher and lower molecular weight molecules is described. It combines in a first dimension either a contour-clamped homogeneous electric field or an orthogonal field alternation gel electrophoresis technique followed by a perpendicular field inversion gel electrophoresis (FIGE) in a second dimension. A complex migration curve after the FIGE run is obtained depending on the applied pulse time, the forward/reverse ratio being kept constant at 3. However, a part of the curve appears as a straight line where the migration is inversely proportional to the molecular weight of the DNA fragments. In this zone, DNA molecules are particularly well separated from other fragments. When the forward pulse time increases, this part is displaced toward the higher molecular weights. Moreover a simple relationship between the middle part of the straight line and the forward pulse time has been established.
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
January 1996,
Electrophoresis,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
October 2017,
Electrophoresis,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
January 1979,
Cell,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
May 1990,
Proceedings of the National Academy of Sciences of the United States of America,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
January 1999,
Methods in molecular biology (Clifton, N.J.),
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
January 2016,
Methods in molecular biology (Clifton, N.J.),
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
July 1992,
Analytical biochemistry,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
March 1986,
Somatic cell and molecular genetics,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
January 1987,
Cancer investigation,
P Sanséau, and
C Tiffoche, and
A Kahloun, and
O Collin, and
J P Rolland, and
J P Le Pennec
February 1974,
The Journal of biological chemistry,
