BIOMAT Database Logo BIOMAT Database Logo

Determination of methamphetamine, amphetamine and piperidine in human urine by high-performance liquid chromatography with chemiluminescence detection. 1990

K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Japan.

A high-performance liquid chromatographic method for the determination of trace levels of methamphetamine, amphetamine and piperidine in human urine is reported. The three compounds, extracted into diethyl ether from alkaline urine, were derivatized with dansyl chloride, then separated on a reversed-phase column and detected by chemiluminescence after reaction with bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide. The corresponding peaks obtained from human urine were identified as the dansyl derivatives by mass spectrometry. Methamphetamine levels as low as 2 x 10(-10) M in urine were determined. The sensitivity of the method is higher than that of Simon's reagent test and gas chromatography.

UI MeSH Term Description Entries
D008163 Luminescent Measurements Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE. Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D008694 Methamphetamine A central nervous system stimulant and sympathomimetic with actions and uses similar to DEXTROAMPHETAMINE. The smokable form is a drug of abuse and is referred to as crank, crystal, crystal meth, ice, and speed. Deoxyephedrine,Desoxyephedrine,Desoxyn,Madrine,Metamfetamine,Methamphetamine Hydrochloride,Methylamphetamine,N-Methylamphetamine,Hydrochloride, Methamphetamine,N Methylamphetamine
D010880 Piperidines A family of hexahydropyridines.
D002849 Chromatography, Gas Fractionation of a vaporized sample as a consequence of partition between a mobile gaseous phase and a stationary phase held in a column. Two types are gas-solid chromatography, where the fixed phase is a solid, and gas-liquid, in which the stationary phase is a nonvolatile liquid supported on an inert solid matrix. Chromatography, Gas-Liquid,Gas Chromatography,Chromatographies, Gas,Chromatographies, Gas-Liquid,Chromatography, Gas Liquid,Gas Chromatographies,Gas-Liquid Chromatographies,Gas-Liquid Chromatography
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000661 Amphetamine A powerful central nervous system stimulant and sympathomimetic. Amphetamine has multiple mechanisms of action including blocking uptake of adrenergics and dopamine, stimulation of release of monamines, and inhibiting monoamine oxidase. Amphetamine is also a drug of abuse and a psychotomimetic. The l- and the d,l-forms are included here. The l-form has less central nervous system activity but stronger cardiovascular effects. The d-form is DEXTROAMPHETAMINE. Desoxynorephedrin,Levoamphetamine,Phenopromin,l-Amphetamine,Amfetamine,Amphetamine Sulfate,Amphetamine Sulfate (2:1),Centramina,Fenamine,Mydrial,Phenamine,Thyramine,levo-Amphetamine,Sulfate, Amphetamine,l Amphetamine,levo Amphetamine

Related Publications

K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of conjugated bile acids in human urine by high-performance liquid chromatography with chemiluminescence detection.
June 1992, Journal of chromatography,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of naproxen in human urine by high-performance liquid chromatography with direct electrogenerated chemiluminescence detection.
August 2009, Talanta,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of amphetamine in human urine by dansyl derivatization and high-performance liquid chromatography with fluorescence detection.
November 1996, Journal of chromatography. B, Biomedical applications,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Simultaneous determination of methamphetamine and its metabolites in the urine samples of abusers by high performance liquid chromatography with chemiluminescence detection.
September 1993, Biological & pharmaceutical bulletin,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of myoglobin in human serum by high-performance liquid chromatography with chemiluminescence detection.
April 1987, Journal of chromatography,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
[Detecting methamphetamine and amphetamine with high performance liquid chromatography].
November 2007, Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Melatonin determination in human urine by high-performance liquid chromatography with fluorescence detection.
April 1986, Journal of chromatography,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of atmospheric nitrobenzanthrones by high-performance liquid chromatography with chemiluminescence detection.
January 2004, Analytical sciences : the international journal of the Japan Society for Analytical Chemistry,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of polyphenols by high-performance liquid chromatography with inhibited chemiluminescence detection.
September 1999, Journal of chromatography. A,
K Hayakawa, and N Imaizumi, and H Ishikura, and E Minogawa, and N Takayama, and H Kobayashi, and M Miyazaki
Determination of maprotiline in plasma by high-performance liquid chromatography with chemiluminescence detection.
July 1995, Journal of chromatography. B, Biomedical applications,
Copied contents to your clipboard!