The aim of this study was to determine the pharmacokinetics of dexamethasone in broiler chickens. Dexamethasone sodium phosphate (0.3mg/kg bodyweight) was injected IV or IM and blood samples were collected at 0, 0.25, 0.5, 0.75, 1, 2, 4, 6, 8, 10, 12 and 24h after administration. Dexamethasone in the plasma samples was measured using a liquid chromatography-tandem mass spectrometry method and the pharmacokinetics analysed according to a one-compartmental model. The maximum plasma concentration after IM administration occurred at 0.37h. The elimination half-life for dexamethasone was 0.46h and 0.70h following IV and IM administration, respectively, which was shorter than other species, while the clearance (1.26L/hkg) was higher than has been reported for other species (<0.5L/hkg). The volume of distribution (∼1L/kg) was similar to values reported for other species and the bioavailability of dexamethasone after IM administration was 100%. The results from this study will be useful in investigating whether inflammatory disease may affect the pharmacokinetic parameters of dexamethasone in chickens.