Biomaterial Database

Electrochemiluminescent lead biosensor based on GR-5 lead-dependent DNAzyme for Ru(phen)3(2+) intercalation and lead recognition. 2013

Ai Gao, and Chun-Xia Tang, and Xi-Wen He, and Xue-Bo Yin

State Key Laboratory of Medicinal Chemical Biology, College of Chemistry, Nankai University, Tianjin, 300071, P.R. China.

An electrochemiluminescent (ECL) lead biosensor was developed based on GR-5 lead-dependent DNAzyme for lead recognition and intercalated ruthenium tris(1,10-phenanthroline) (Ru(phen)(3)(2+)) as the ECL probe. The thiol-modified substrate was first immobilized on the surface of the gold electrode via gold-sulfur self-assembly. Subsequently, the hybridization of DNAzyme and its substrate and the automatic intercalation of Ru(phen)(3)(2+) proceeded. Intercalated Ru(phen)(3)(2+) can transfer electrons through double-stranded DNA to the electrode and its electrochemiluminescence was excited with a potential step using tripropylamine as the coreactant. In the presence of lead, the substrate cleaves at the scissile ribo-adenine into two fragments. The dissociation of DNAzyme occurs, leading to the releasing of intercalated Ru(phen)(3)(2+) accompanied by a decrease in the intensity of electrochemiluminescence. A quantity of lead can be calculated from this decrease. The biosensor is highly sensitive and specific, along with an ultra-low limit of detection of 0.9 pM and a dynamic range from 2 to 1000 pM. It enables analysis of trace amounts of lead in serum samples. The combination of the intercalated-Ru(phen)(3)(2+) ECL probe and the cofactor-dependent DNAzyme may push the performance of cofactor-sensing tactics to the extreme.

UI	MeSH Term	Description	Entries
D007854	Lead	A soft, grayish metal with poisonous salts; atomic number 82, atomic weight 207.2, symbol Pb.
D008163	Luminescent Measurements	Techniques used for determining the values of photometric parameters of light resulting from LUMINESCENCE.	Bioluminescence Measurements,Bioluminescent Assays,Bioluminescent Measurements,Chemiluminescence Measurements,Chemiluminescent Assays,Chemiluminescent Measurements,Chemoluminescence Measurements,Luminescence Measurements,Luminescent Assays,Luminescent Techniques,Phosphorescence Measurements,Phosphorescent Assays,Phosphorescent Measurements,Assay, Bioluminescent,Assay, Chemiluminescent,Assay, Luminescent,Assay, Phosphorescent,Assays, Bioluminescent,Assays, Chemiluminescent,Assays, Luminescent,Assays, Phosphorescent,Bioluminescence Measurement,Bioluminescent Assay,Bioluminescent Measurement,Chemiluminescence Measurement,Chemiluminescent Assay,Chemiluminescent Measurement,Chemoluminescence Measurement,Luminescence Measurement,Luminescent Assay,Luminescent Measurement,Luminescent Technique,Measurement, Bioluminescence,Measurement, Bioluminescent,Measurement, Chemiluminescence,Measurement, Chemiluminescent,Measurement, Chemoluminescence,Measurement, Luminescence,Measurement, Luminescent,Measurement, Phosphorescence,Measurement, Phosphorescent,Measurements, Bioluminescence,Measurements, Bioluminescent,Measurements, Chemiluminescence,Measurements, Chemiluminescent,Measurements, Chemoluminescence,Measurements, Luminescence,Measurements, Luminescent,Measurements, Phosphorescence,Measurements, Phosphorescent,Phosphorescence Measurement,Phosphorescent Assay,Phosphorescent Measurement,Technique, Luminescent,Techniques, Luminescent
D009942	Organometallic Compounds	A class of compounds of the type R-M, where a C atom is joined directly to any other element except H, C, N, O, F, Cl, Br, I, or At. (Grant & Hackh's Chemical Dictionary, 5th ed)	Metallo-Organic Compound,Metallo-Organic Compounds,Metalloorganic Compound,Organometallic Compound,Metalloorganic Compounds,Compound, Metallo-Organic,Compound, Metalloorganic,Compound, Organometallic,Compounds, Metallo-Organic,Compounds, Metalloorganic,Compounds, Organometallic,Metallo Organic Compound,Metallo Organic Compounds
D010084	Oxidation-Reduction	A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).	Redox,Oxidation Reduction
D010618	Phenanthrolines		Phenanthroline
D011437	Propylamines	Derivatives of propylamine (the structural formula NH2CH2CH2CH3).
D004563	Electrochemistry	The study of chemical changes resulting from electrical action and electrical activity resulting from chemical changes.	Electrochemistries
D001483	Base Sequence	The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.	DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D015374	Biosensing Techniques	Any of a variety of procedures which use biomolecular probes to measure the presence or concentration of biological molecules, biological structures, microorganisms, etc., by translating a biochemical interaction at the probe surface into a quantifiable physical signal.	Bioprobes,Biosensors,Electrodes, Enzyme,Biosensing Technics,Bioprobe,Biosensing Technic,Biosensing Technique,Biosensor,Electrode, Enzyme,Enzyme Electrode,Enzyme Electrodes,Technic, Biosensing,Technics, Biosensing,Technique, Biosensing,Techniques, Biosensing
D057230	Limit of Detection	Concentration or quantity that is derived from the smallest measure that can be detected with reasonable certainty for a given analytical procedure.	Limits of Detection,Detection Limit,Detection Limits