Biomaterial Database

Surface carbohydrate differences of Glossina salivary glands and infectivity of Trypanosoma brucei gambiense to Glossina. 1990

C J Okolo, and L Jenni, and D H Molyneux, and K R Wallbanks

Department of Biological Sciences, University of Salford, UK.

Incubation of fluorescein- and biotin-lectin conjugates with the salivary glands of Glossina spp has revealed inter- and intraspecific variation in the surface carbohydrates of the glands. The degree of Con A binding to the basal laminae of the glands of the two Glossina palpalis subspecies, G.p. palpalis and G.p. gambiensis was markedly different. The infectivity of T.b. gambiense sensu lato isolates to G.p. palpalis and G.p. gambiensis was compared. G.p. gambiensis from the field and from laboratory colonies transmitted T.b. gambiense sensu lato isolated from patients in Ivory Coast whereas G.p. palpalis appears totally refractory to all T.b. gambiense isolates used. Although the relationship between the surface of the basal laminae of the salivary gland exposed to the haemocoele and trypanosome infection is not known the consistent differences observed in lectin binding to the salivary glands suggest that differences in basic physiology of the glands exist which might correlate with susceptibility to trypanosome infection.

UI	MeSH Term	Description	Entries
D008856	Microscopy, Fluorescence	Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.	Fluorescence Microscopy,Immunofluorescence Microscopy,Microscopy, Immunofluorescence,Fluorescence Microscopies,Immunofluorescence Microscopies,Microscopies, Fluorescence,Microscopies, Immunofluorescence
D002241	Carbohydrates	A class of organic compounds composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n. The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES.	Carbohydrate
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012469	Salivary Glands	Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).	Gland, Salivary,Glands, Salivary,Salivary Gland
D014347	Trypanosoma brucei gambiense	A hemoflagellate subspecies of parasitic protozoa that causes Gambian or West African sleeping sickness in humans. The vector host is usually the tsetse fly (Glossina).	Trypanosoma gambiense,Trypanosoma brucei gambienses,Trypanosoma gambienses,brucei gambiense, Trypanosoma,brucei gambienses, Trypanosoma,gambiense, Trypanosoma brucei,gambienses, Trypanosoma
D014370	Tsetse Flies	Bloodsucking flies of the genus Glossina, found primarily in equatorial Africa. Several species are intermediate hosts of trypanosomes.	Glossina,Flies, Tsetse,Fly, Tsetse,Glossinas,Tsetse Fly
D037102	Lectins	Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.	Animal Lectin,Animal Lectins,Isolectins,Lectin,Isolectin,Lectin, Animal,Lectins, Animal