Biomaterial Database

Infectivity reacquisition by Trypanosoma brucei brucei cultivated with tsetse salivary glands. 1977

I Cunningham, and B M Honigberg

Reacquisition of infectivity four mice was observed in cultures of Trypanosoma brucei brucei grown in the presence of tests fly salivary gland explants in a medium based on the amino acid composition of Glossina hemolymph and containing fetal bovine serum. High infection rates were obtained in mice inoculated with about 1.5 X 10(8) organisms. Infectivity reacquisition was correlated with invasion of the salivary glands by the parasites. Few small trypanosomes with subterminal kinetoplasts (metacyclic-like forms) were found in the infective inoculums. The parasitemias in mice consisted of pleomorphic cultivable trypanosomes. Cultures initiated by these organisms and then placed with the head-salivary gland preparations became infective for mice.

UI	MeSH Term	Description	Entries
D009924	Organ Culture Techniques	A technique for maintenance or growth of animal organs in vitro. It refers to three-dimensional cultures of undisaggregated tissue retaining some or all of the histological features of the tissue in vivo. (Freshney, Culture of Animal Cells, 3d ed, p1)	Organ Culture,Culture Technique, Organ,Culture Techniques, Organ,Organ Culture Technique,Organ Cultures
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D012469	Salivary Glands	Glands that secrete SALIVA in the MOUTH. There are three pairs of salivary glands (PAROTID GLAND; SUBLINGUAL GLAND; SUBMANDIBULAR GLAND).	Gland, Salivary,Glands, Salivary,Salivary Gland
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor
D014346	Trypanosoma brucei brucei	A hemoflagellate subspecies of parasitic protozoa that causes nagana in domestic and game animals in Africa. It apparently does not infect humans. It is transmitted by bites of tsetse flies (Glossina).	Trypanosoma brucei,Trypanosoma brucei bruceus,Trypanosoma bruceus,brucei brucei, Trypanosoma,brucei, Trypanosoma brucei,bruceus, Trypanosoma,bruceus, Trypanosoma brucei
D014352	Trypanosomiasis	Infection with protozoa of the genus TRYPANOSOMA.	Trypanosomiases
D014370	Tsetse Flies	Bloodsucking flies of the genus Glossina, found primarily in equatorial Africa. Several species are intermediate hosts of trypanosomes.	Glossina,Flies, Tsetse,Fly, Tsetse,Glossinas,Tsetse Fly
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus