-transparent.png){kind=logo}
Nucleotide sequences of VJ (variable-joining) junctional regions of V14+ alpha-chain T-cell receptor genes show that most V alpha 14+ T cells use one alpha chain (V alpha 14J alpha 281 with a one-nucleotide N region, which is frequently used in keyhole limpet hemocyanin-specific suppressor T-cell hybridomas) in unprimed mice. Moreover, the frequency of this alpha-chain expression was greater than 1.5% of the total alpha chains found in laboratory strains, including B10 congenic mice. This is about 10(4) times higher than was expected. The V14J281 alpha-chain expression was relatively low but was significant in CD4+/CD8+ immature thymocytes and became quite high in mature single-positive T cells, implying that this alpha chain is selected during T-cell maturation. V14J281 expression increased with time after birth and reached a maximum at around 5 weeks of age. The ligand seems to be a self molecule and to be present in laboratory strains but to be absent in a wild mouse, Mus musculus molossinus, because bone marrow chimeras clearly showed that bone marrow cells derived from Mus musculus molossinus negative for this alpha chain raised V14J281-positive T cells in a C57BL/6 environment. The above results suggest that there are some selection mechanisms for this cell type other than those for conventional alpha beta T cells and also that the homogenous VJ junction of the V14J281 alpha chain plays a pivotal role in the selection of the T cell and its ligand reactivity.
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
January 1990,
Immunogenetics,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
November 1986,
Proceedings of the National Academy of Sciences of the United States of America,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
March 1992,
European journal of immunology,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
April 1992,
Arerugi = [Allergy],
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
October 1988,
Journal of autoimmunity,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
June 1995,
Journal of neuroimmunology,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
November 1987,
Proceedings of the National Academy of Sciences of the United States of America,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
September 1991,
European journal of immunology,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
September 2005,
Fish & shellfish immunology,
H Koseki, and
K Imai, and
F Nakayama, and
T Sado, and
K Moriwaki, and
M Taniguchi
December 1988,
Molecular and cellular biology,
