Detection of low-frequency HIV type 1 reverse transcriptase drug resistance mutations by ultradeep sequencing in naive HIV type 1-infected individuals. 2014

Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
1 Laboratoire MFP UMR 5234, Université Bordeaux Segalen , Bordeaux, France .

Genotypic resistance testing is recommended to evaluate the susceptibility of HIV to antiretroviral drugs. These tests are based on bulk population sequencing and thus consider only variants representing more than 20% of the viral population, whereas next generation sequencing methods allow detection below this threshold. We aimed to evaluate the potential use of ultradeep pyrosequencing (UDPS) for genotypic resistance testing in clinical routine at the University Hospital of Bordeaux, France. We performed UDPS on reverse transcriptase (RT) from 47 HIV-1 individuals, naive of antiretroviral treatment and for whom genotypic resistance testing was requested for clinical management in 2011-2012. In 8.5% of the patients, only low-frequency variants harboring RT drug resistance mutations were detected raising the question of their clinical significance. Rilpivirine-associated resistance mutations were detected in 19.1% of our population study. To conclude, UDPS could become a routine tool for the evaluation of HIV-infected patients in hospital laboratories.

UI MeSH Term Description Entries
D005602 France A country in western Europe bordered by the Atlantic Ocean, the English Channel, the Mediterranean Sea, and the countries of Belgium, Germany, Italy, Spain, Switzerland, the principalities of Andorra and Monaco, and by the duchy of Luxembourg. Its capital is Paris. Corsica,Saint Pierre and Miquelon,Miquelon and Saint Pierre,Miquelon and St. Pierre,St. Pierre and Miquelon
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D015497 HIV-1 The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte. Human immunodeficiency virus 1,HIV-I,Human Immunodeficiency Virus Type 1,Immunodeficiency Virus Type 1, Human
D015658 HIV Infections Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS). HTLV-III Infections,HTLV-III-LAV Infections,T-Lymphotropic Virus Type III Infections, Human,HIV Coinfection,Coinfection, HIV,Coinfections, HIV,HIV Coinfections,HIV Infection,HTLV III Infections,HTLV III LAV Infections,HTLV-III Infection,HTLV-III-LAV Infection,Infection, HIV,Infection, HTLV-III,Infection, HTLV-III-LAV,Infections, HIV,Infections, HTLV-III,Infections, HTLV-III-LAV,T Lymphotropic Virus Type III Infections, Human
D054303 HIV Reverse Transcriptase A reverse transcriptase encoded by the POL GENE of HIV. It is a heterodimer of 66 kDa and 51 kDa subunits that are derived from a common precursor protein. The heterodimer also includes an RNAse H activity (RIBONUCLEASE H, HUMAN IMMUNODEFICIENCY VIRUS) that plays an essential role the viral replication process. Reverse Transcriptase, HIV,Reverse Transcriptase, Human Immunodeficiency Virus,Transcriptase, HIV Reverse
D059014 High-Throughput Nucleotide Sequencing Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc. High-Throughput Sequencing,Illumina Sequencing,Ion Proton Sequencing,Ion Torrent Sequencing,Next-Generation Sequencing,Deep Sequencing,High-Throughput DNA Sequencing,High-Throughput RNA Sequencing,Massively-Parallel Sequencing,Pyrosequencing,DNA Sequencing, High-Throughput,High Throughput DNA Sequencing,High Throughput Nucleotide Sequencing,High Throughput RNA Sequencing,High Throughput Sequencing,Massively Parallel Sequencing,Next Generation Sequencing,Nucleotide Sequencing, High-Throughput,RNA Sequencing, High-Throughput,Sequencing, Deep,Sequencing, High-Throughput,Sequencing, High-Throughput DNA,Sequencing, High-Throughput Nucleotide,Sequencing, High-Throughput RNA,Sequencing, Illumina,Sequencing, Ion Proton,Sequencing, Ion Torrent,Sequencing, Massively-Parallel,Sequencing, Next-Generation
D020125 Mutation, Missense A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed) Missense Mutation,Missense Mutations,Mutations, Missense
D024882 Drug Resistance, Viral The ability of viruses to resist or to become tolerant to chemotherapeutic agents or antiviral agents. This resistance is acquired through gene mutation. Antiviral Drug Resistance,Antiviral Drug Resistances,Drug Resistances, Viral

Related Publications

Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
January 2011, East African medical journal,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
January 2020, PloS one,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
April 2011, AIDS research and human retroviruses,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
November 2006, Archives of medical research,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
January 1998, Journal of human virology,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
June 2012, AIDS (London, England),
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
February 2010, Journal of medical virology,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
February 2004, AIDS research and human retroviruses,
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
July 1999, AIDS (London, England),
Pantxika Bellecave, and Patricia Recordon-Pinson, and Jennifer Papuchon, and Marie-Anne Vandenhende, and Sandrine Reigadas, and Brigitte Tauzin, and Hervé Fleury
October 2012, AIDS research and human retroviruses,
Copied contents to your clipboard!