BIOMAT Database Logo BIOMAT Database Logo

Assignment of the vascular smooth muscle actin gene ACTSA to human chromosome 10. 1990

H Ueyama, and G Bruns, and N Kanda
Department of Medical Biochemistry, Shiga University of Medical Science, Otsu, Japan.

Human vascular smooth muscle actin gene (ACTSA) was cloned and its unique sequence was used as the hybridization probe for Southern blot analysis of DNAs from 18 rodent-human somatic cell hybrids; the gene was assigned to human chromosome 10. Regional mapping by in situ hybridization showed that the gene is located on the long arm (q22-q24) of the chromosome. Thus, the gene is on a different chromosome from the other four actin genes so far examined.

UI MeSH Term Description Entries
D009130 Muscle, Smooth Unstriated and unstriped muscle, one of the muscles of the internal organs, blood vessels, hair follicles, etc. Contractile elements are elongated, usually spindle-shaped cells with centrally located nuclei. Smooth muscle fibers are bound together into sheets or bundles by reticular fibers and frequently elastic nets are also abundant. (From Stedman, 25th ed) Muscle, Involuntary,Smooth Muscle,Involuntary Muscle,Involuntary Muscles,Muscles, Involuntary,Muscles, Smooth,Smooth Muscles
D002879 Chromosomes, Human, Pair 10 A specific pair of GROUP C CHROMOSOMES of the human chromosome classification. Chromosome 10
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000199 Actins Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle. F-Actin,G-Actin,Actin,Isoactin,N-Actin,alpha-Actin,alpha-Isoactin,beta-Actin,gamma-Actin,F Actin,G Actin,N Actin,alpha Actin,alpha Isoactin,beta Actin,gamma Actin
D015183 Restriction Mapping Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA. Endonuclease Mapping, Restriction,Enzyme Mapping, Restriction,Site Mapping, Restriction,Analysis, Restriction Enzyme,Enzyme Analysis, Restriction,Restriction Enzyme Analysis,Analyses, Restriction Enzyme,Endonuclease Mappings, Restriction,Enzyme Analyses, Restriction,Enzyme Mappings, Restriction,Mapping, Restriction,Mapping, Restriction Endonuclease,Mapping, Restriction Enzyme,Mapping, Restriction Site,Mappings, Restriction,Mappings, Restriction Endonuclease,Mappings, Restriction Enzyme,Mappings, Restriction Site,Restriction Endonuclease Mapping,Restriction Endonuclease Mappings,Restriction Enzyme Analyses,Restriction Enzyme Mapping,Restriction Enzyme Mappings,Restriction Mappings,Restriction Site Mapping,Restriction Site Mappings,Site Mappings, Restriction
D015342 DNA Probes Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections. Chromosomal Probes,DNA Hybridization Probe,DNA Probe,Gene Probes, DNA,Conserved Gene Probes,DNA Hybridization Probes,Whole Chromosomal Probes,Whole Genomic DNA Probes,Chromosomal Probes, Whole,DNA Gene Probes,Gene Probes, Conserved,Hybridization Probe, DNA,Hybridization Probes, DNA,Probe, DNA,Probe, DNA Hybridization,Probes, Chromosomal,Probes, Conserved Gene,Probes, DNA,Probes, DNA Gene,Probes, DNA Hybridization,Probes, Whole Chromosomal

Related Publications

H Ueyama, and G Bruns, and N Kanda
Angiotensin II regulates human vascular smooth muscle alpha-actin gene expression.
October 1993, Biochemical and biophysical research communications,
H Ueyama, and G Bruns, and N Kanda
Assignment of the vascular endothelial growth factor gene to human chromosome 6p21.3.
April 1996, Circulation,
H Ueyama, and G Bruns, and N Kanda
The mouse smooth muscle gamma actin gene is on chromosome 6.
May 1990, Somatic cell and molecular genetics,
H Ueyama, and G Bruns, and N Kanda
Complete sequence of human vinculin and assignment of the gene to chromosome 10.
August 1990, Proceedings of the National Academy of Sciences of the United States of America,
H Ueyama, and G Bruns, and N Kanda
Assignment of the human skeletal muscle [FC12]a-actin gene (ACTA1) to chromosome 1q42.13-->q42.2 by radiation hybrid mapping.
January 1998, Cytogenetics and cell genetics,
H Ueyama, and G Bruns, and N Kanda
Assignment of estradiol receptor gene to mouse chromosome 10.
November 1988, Journal of steroid biochemistry,
H Ueyama, and G Bruns, and N Kanda
Interleukin-10 inhibits human vascular smooth muscle proliferation.
April 1998, Journal of molecular and cellular cardiology,
H Ueyama, and G Bruns, and N Kanda
Divergent structure of the human synexin (annexin VII) gene and assignment to chromosome 10.
June 1994, Biochemistry,
H Ueyama, and G Bruns, and N Kanda
Nuclear Smooth Muscle α-actin in Vascular Smooth Muscle Cell Differentiation.
February 2023, Research square,
H Ueyama, and G Bruns, and N Kanda
Assignment of the gene for human sphingolipid activator protein-2 (SAP-2) to chromosome 10.
July 1985, American journal of human genetics,
Copied contents to your clipboard!