-transparent.png){kind=logo}
When CEM cells were incubated with [3H]-labeled 3'-azido-2',3'-dideoxyguanosine (AzddGuo), the isotope was largely recovered as ribo- and deoxyribonucleotides in the acid soluble fraction of CEM cells, due to extensive catabolism of AzddGuo and recycling of the guanine formed by purine nucleoside phosphorylase. Only 10% was found as the AzddGuo nucleotides, with the diphosphate of AzddGuo as the dominating nucleotide at all time points. Thus nucleoside diphosphate kinase was rate limiting for the formation of AzddGuo triphosphate, responsible for the toxic and antiviral activity of the nucleoside. Inhibition of de novo deoxyribonucleotide synthesis with hydroxyurea increased the phosphorylation of AzddGuo twofold.
A Karlsson, and
P Reichard, and
F Eckstein
January 2004,
Nucleosides, nucleotides & nucleic acids,
A Karlsson, and
P Reichard, and
F Eckstein
January 2014,
Antiviral research,
A Karlsson, and
P Reichard, and
F Eckstein
January 1987,
Lancet (London, England),
A Karlsson, and
P Reichard, and
F Eckstein
January 2008,
Nucleic acids symposium series (2004),
A Karlsson, and
P Reichard, and
F Eckstein
January 2009,
Nucleic acids symposium series (2004),
A Karlsson, and
P Reichard, and
F Eckstein
June 1987,
Biochemical and biophysical research communications,
A Karlsson, and
P Reichard, and
F Eckstein
November 1991,
The Journal of infectious diseases,
A Karlsson, and
P Reichard, and
F Eckstein
November 1992,
The Journal of biological chemistry,
A Karlsson, and
P Reichard, and
F Eckstein
January 2000,
Nucleosides, nucleotides & nucleic acids,
A Karlsson, and
P Reichard, and
F Eckstein
December 1999,
Molecular pharmacology,
