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We tested the effect of interleukin 1 (IL-1) on the growth of leukemic blast progenitors from patients with acute myeloblastic leukemia (AML). A purified blast cell fraction depleted of both T cells and phagocytic cells was tested at different cell densities. Addition of 1 ng/ml of IL-1 alpha alone enhanced blast colony formation in 10 of 13 cases tested, and the enhancement was prominent when plated cell densities were lowered. The conditioned media (CM) from AML patients contained varied levels of IL-1 activity, and following depletion of phagocytic cells, the levels decreased markedly in all cases tested. Addition of either antiserum against IL-1 alpha or IL-1 beta reduced the IL-1 activity in CM, suggesting that AML blasts produce both IL-1 alpha and IL-1 beta. Addition of IL-1 alpha or IL-1 beta antiserum inhibited blast colony formation in a dose-dependent manner, and a combination of both antisera showed the most marked inhibition. However, the augmentation of blast colony formation was almost completely inhibited by addition of anti-granulocyte-macrophage colony-stimulating factor (GM-CSF) serum in all three cases tested. IL-1 is also devoid of this activity when tested in the presence of a combination of granulocyte CSF (G-CSF), GM-CSF, and interleukin 3 (IL-3) at an optimal concentration. These results suggest that blast cells could produce and secrete CSF(s) and/or IL-1, and that the growth-enhancing effect of IL-1 on AML blasts is indirect, via production of CSFs by leukemic cells.
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
January 1986,
Leukemia research,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
June 1985,
Blood,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
December 1990,
Cancer research,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
February 1987,
Blood,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
September 1978,
Blood,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
January 1992,
Acta haematologica,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
May 1988,
Cancer research,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
October 1987,
Experimental hematology,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
September 1991,
Journal of cellular physiology,
I Murohashi, and
S Tohda, and
T Suzuki, and
K Nagata, and
Y Yamashita, and
N Nara
March 1986,
Blood,
