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The action of iron-bleomycin and O2 in cleaving DNA has been resolved into two kinetic events following the initial attack on DNA by the kinetically competent drug species, "activated bleomycin." At 4 degrees C, DNA strand scission, monitored both viscometrically and fluorimetrically (t1/2 = 2.5-5 min), precedes the release from DNA of nucleic base propenals, which is half complete in about 40 min. Therefore, a moderately stable intermediate consisting of cleaved DNA bearing a base propenal precursor is formed. The release of tritium from deoxyribose carbon-2 occurs at the time of DNA scission, which is consistent with the base propenal precursor retaining the deoxyribose-3'-phosphate bond. Specific mechanistic proposals are discussed.
R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
August 1981,
The Journal of biological chemistry,
R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
October 1994,
The Journal of biological chemistry,
R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
July 1982,
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R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
January 1998,
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R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
May 1989,
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R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
January 1985,
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R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
September 1985,
Biochemistry,
R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
December 1994,
International journal of radiation biology,
R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
April 1993,
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R M Burger, and
S J Projan, and
S B Horwitz, and
J Peisach
November 1986,
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