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The molecular basis for the expression of rat embryonic fibroblast tropomyosin 1 and skeletal muscle beta-tropomyosin was determined. cDNA clones encoding these tropomyosin isoforms exhibit complete identity except for two carboxy-proximal regions (amino acids 189 to 213 and 258 to 284) and different 3'-untranslated sequences. The isoform-specific regions delineate the troponin T-binding domains of skeletal muscle tropomyosin. Analysis of genomic clones indicates that there are two separate loci in the rat genome that contain sequences complementary to these mRNAs. One locus is a pseudogene. The other locus contains a single gene made up of 11 exons and spans approximately 10 kilobases. Sequences common to all mRNAs were found in exons 1 through 5 (amino acids 1 to 188) and exons 8 and 9 (amino acids 214 to 257). Exons 6 and 11 are specific for fibroblast mRNA (amino acids 189 to 213 and 258 to 284, respectively), while exons 7 and 10 are specific for skeletal muscle mRNA (amino acids 189 to 213 and 258 to 284, respectively). In addition, exons 10 and 11 each contain the entire 3'-untranslated sequences of the respective mRNAs including the polyadenylation site. Although the gene is also expressed in smooth muscle (stomach, uterus, and vas deferens), only the fibroblast-type splice products can be detected in these tissues. S1 and primer extension analyses indicate that all mRNAs expressed from this gene are transcribed from a single promoter. The promoter was found to contain G-C-rich sequences, a TATA-like sequence TTTTA, no identifiable CCAAT box, and two putative Sp1-binding sites.
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
December 1994,
Proceedings of the National Academy of Sciences of the United States of America,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
August 1987,
The Journal of biological chemistry,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
October 1990,
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
April 1990,
Molecular and cellular biology,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
February 1992,
Journal of muscle research and cell motility,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
December 1984,
Molecular and cellular biology,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
February 1988,
Molecular and cellular biology,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
August 1992,
Blood,
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
December 1998,
Molecular endocrinology (Baltimore, Md.),
D M Helfman, and
S Cheley, and
E Kuismanen, and
L A Finn, and
Y Yamawaki-Kataoka
April 1995,
Gene,
