BIOMAT Database Logo BIOMAT Database Logo

T-2 toxin and its metabolite HT-2 toxin combined with insulin-like growth factor-I modify progesterone secretion by porcine ovarian granulosa cells. 2014

Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
a Department of Animal Physiology, Faculty of Biotechnology and Food Sciences , Slovak University of Agriculture in Nitra , Nitra , Slovak Republic.

The aim of this study was to examine the effect of A-trichothecenes T-2 and HT-2 toxins combined with insulin-like growth factor I (IGF-I) on the release of steroid hormone progesterone (P4) by porcine ovarian granulosa cells (GCs). The cells were incubated without (control) or with treatments of A-trichothecenes T-2 (100 and 1000 ng/mL)/ HT-2 (100 and 1000 ng/mL) combined with IGF-I (1, 10 and 100 ng/mL) for 24 h. Progesterone secretion was determined by RIA. The release of P4 by GCs after addition of T-2 toxin (at 100 ng/mL) combined with IGF-I (at 10 but not at 1 and 100 ng/mL) and HT-2 toxin (at 100 ng/mL) combined with IGF-I (at all doses) was significantly (P < 0.05) inhibited. On the other hand the release of P4 after addition of T-2/ HT-2 toxin (at 1000 ng/mL) combined with IGF-I (at all doses) was significantly (P < 0.05) stimulated. Alone IGF-I addition (at 10, 100 but not at 1 ng/mL) significantly (P < 0.05) stimulated P4 release by GCs. The results of our in vitro study indicate the T-2 and HT-2 toxins combined with IGF-I could modify progesterone secretion by porcine ovarian granulosa cells and potentially regulate process of steroidogenesis in the ovaries. Currently, occurrence of mycotoxins in food and feed is a worldwide problem and therefore study of these toxins as well as their interaction with different substances such as growth factors could be beneficial for better understanding of mechanism of their toxic effects in organism.

UI MeSH Term Description Entries
D007334 Insulin-Like Growth Factor I A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor. IGF-I,Somatomedin C,IGF-1,IGF-I-SmC,Insulin Like Growth Factor I,Insulin-Like Somatomedin Peptide I,Insulin Like Somatomedin Peptide I
D011374 Progesterone The major progestational steroid that is secreted primarily by the CORPUS LUTEUM and the PLACENTA. Progesterone acts on the UTERUS, the MAMMARY GLANDS and the BRAIN. It is required in EMBRYO IMPLANTATION; PREGNANCY maintenance, and the development of mammary tissue for MILK production. Progesterone, converted from PREGNENOLONE, also serves as an intermediate in the biosynthesis of GONADAL STEROID HORMONES and adrenal CORTICOSTEROIDS. Pregnenedione,Progesterone, (13 alpha,17 alpha)-(+-)-Isomer,Progesterone, (17 alpha)-Isomer,Progesterone, (9 beta,10 alpha)-Isomer
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005260 Female Females
D006107 Granulosa Cells Supporting cells for the developing female gamete in the OVARY. They are derived from the coelomic epithelial cells of the gonadal ridge. Granulosa cells form a single layer around the OOCYTE in the primordial ovarian follicle and advance to form a multilayered cumulus oophorus surrounding the OVUM in the Graafian follicle. The major functions of granulosa cells include the production of steroids and LH receptors (RECEPTORS, LH). Cell, Granulosa,Cells, Granulosa,Granulosa Cell
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D013552 Swine Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA). Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D013605 T-2 Toxin A potent mycotoxin produced in feedstuffs by several species of the genus FUSARIUM. It elicits a severe inflammatory reaction in animals and has teratogenic effects. Fusaritoxin,Fusariotoxin,T-2 Toxin, alpha,4 beta-Isomer,T2 Toxin,T 2 Toxin,Toxin, T-2,Toxin, T2

Related Publications

Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Concomitant effects of growth hormone on secretion of insulin-like growth factor I and progesterone by cultured porcine granulosa cells.
October 1987, Endocrinology,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Presumptive mediators of growth hormone action on insulin-like growth factor I release by porcine ovarian granulosa cells.
January 2000, Biological signals and receptors,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Adiponectin increases insulin-like growth factor I-induced progesterone and estradiol secretion in human granulosa cells.
December 2009, Fertility and sterility,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Melatonin and serotonin directly regulate oxytocin, insulin-like growth factor-I and progesterone secretion by cultured human granulosa cells.
January 1995, Advances in experimental medicine and biology,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Growth factors regulate immunoreactive insulin-like growth factor-I production by cultured porcine granulosa cells.
July 1988, Endocrinology,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Regulation of insulin-like growth factor I biosynthesis in porcine granulosa cells.
November 1996, Endocrinology,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Insulin-like growth factor-I stimulates oxytocin and progesterone production by bovine granulosa cells in culture.
January 1988, The Journal of endocrinology,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Mechanism of Apoptosis in Porcine Ovarian Granulosa Cells Triggered by T-2 Toxin.
May 2024, Genes,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Insulin-like growth factor-I and follicle-stimulating hormone suppress insulin-like growth factor binding protein-1 secretion by human granulosa-luteal cells.
September 1992, The Journal of clinical endocrinology and metabolism,
Nora Maruniakova, and Attila Kadasi, and Alexander V Sirotkin, and Jozef Bulla, and Adriana Kolesarova
Gonadotropins and estradiol stimulate immunoreactive insulin-like growth factor-I production by porcine granulosa cells in vitro.
January 1987, Endocrinology,
Copied contents to your clipboard!