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A fluorescence assay that detects long branches in the starch polysaccharide amylopectin. 2014

Sophie R Beeren, and Ole Hindsgaul
Carlsberg Laboratory, Gamle Carlsberg Vej 10, Copenhagen V, 1799, Denmark. Sophie.Beeren@carlsberglab.dk Ole.Hindsgaul@carlsberglab.dk.

Long α(1-4)-linked glucopyranose branches in the starch polysaccharide amylopectin can be detected by the specific binding of an anionic amphiphilic fluorescent probe. The probe forms spermidine-stabilised micelles in water resulting in fluorescence quenching. By extracting the probe from the micelles polysaccharides are detected in a "turn-on" fluorescence assay.

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