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Some naturally occurring Nif(+) and Nif(-)uperscript> strains of Bacillus polymyxa and Bacillus macerans were tested for DNA homology to the Klebsiella pneumoniae nif genes by Southern hybridization. Genomic DNA from all Nif(+) strains carried homologous sequences only to structural nif genes. The homology detected was limited to nifH and nifD. The hybridization pattern observed suggested that both genes are contiguous on the chromosome of the Bacillus strains. No homology was found between the genomic DNA from Nif(-)uperscript> strains and the K. pneumoniae nif genes.
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