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Glutathione S-transferases (GSTs) are ubiquitous enzymes in animals and plants, and they are multifunctional proteins encoded by a large gene family. GSTs are involved in response to the oxidative stress including drought, salt, heavy metals, and so on. Under oxidative stress, the excessive reactive oxygen species (ROS) induce an increase in GST levels, and then the GSTs metabolize the toxic products of lipid peroxidation, damaged DNA and other molecules. Previously, a full-length cDNA of a novel zeta GST gene, PpGST, was characterized from fruit of Pyrus pyrifolia Nakai cv Huobali. In the present study, a constitutive plant expression vector of PpGSTwas constructed and transferred into tobacco (Nicotiana tabacum L. cv Xanthi) to verify the function of PpGST. As a result, the PpGSTgene was successfully integrated into the genome of the transgenic tobacco lines and expressed as expected in the transformants through Southern blotting and quantitative reverse transcription-polymerase chain reaction analysis. Growth of T1 generation plants of PpGST transgenic lines and WT under non-stressful conditions was similar, however, the transgenic tobacco lines showed relatively normal growth under drought, NaCl, and cadmium (Cd) stresses. Furthermore, the T1 transgenic tobacco lines showed significantly slower superoxide anion production rate than the WT under abiotic stress. Simultaneously, the MDA content of each T1 transgenic tobacco plant was only slightly increased and significantly lower than that of the WT under drought, salt and Cd stress. Together with the GST activity of the transgenic tobacco lines, which was significantly increased under stressful conditions, as compared with that in WT, overexpression of PpGSTin tobacco enhanced the tolerance of transgenic tobacco lines to oxidative damage caused by drought, NaCl, and Cd stresses.
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
June 2018,
Biochemistry. Biokhimiia,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
September 2019,
Biology open,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
November 2000,
Plant & cell physiology,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
October 2006,
Plant cell reports,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
October 1997,
Nature biotechnology,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
April 2017,
Plant cell reports,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
January 2022,
Frontiers in plant science,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
November 2014,
Plant cell reports,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
April 2019,
Applied biochemistry and biotechnology,
D Liu, and
Y Liu, and
J Rao, and
G Wang, and
H Li, and
F Ge, and
C Chen
August 2003,
The Plant journal : for cell and molecular biology,
