BIOMAT Database Logo BIOMAT Database Logo

A suction blister method that gives epidermal biopsies from mice of different ages, suitable for histologic studies of Langerhans cells. 1987

S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Danish Cancer Society, Department of Tumor Virology, Aarhus.

A modified suction blister device has been developed. It allows in vivo retrieval of 3 x 3 mm2 mouse epidermis biopsies. Separation takes place exactly at the epidermal-dermal junction. Young skin and iso-grafted skin require suction with a lower bar value than skin from young and old non-grafted animals. Langerhans cells in biopsies were identified both with ATPase staining and with monoclonal antibody. Suction blisters produce Langerhans cell counts similar to those obtained on EDTA-separated epidermis.

UI MeSH Term Description Entries
D007801 Langerhans Cells Recirculating, dendritic, antigen-presenting cells containing characteristic racket-shaped granules (Birbeck granules). They are found principally in the stratum spinosum of the EPIDERMIS and are rich in Class II MAJOR HISTOCOMPATIBILITY COMPLEX molecules. Langerhans cells were the first dendritic cell to be described and have been a model of study for other dendritic cells (DCs), especially other migrating DCs such as dermal DCs and INTERSTITIAL DENDRITIC CELLS. Langerhans Cell,Dendritic Cells, Dermal,Dendritic Cells, Epidermal,Dendritic Cells, Skin,Dermal Dendritic Cells,Epidermal Dendritic Cells,Skin Dendritic Cells,Cell, Dermal Dendritic,Cell, Epidermal Dendritic,Cell, Langerhans,Cell, Skin Dendritic,Cells, Dermal Dendritic,Cells, Epidermal Dendritic,Cells, Langerhans,Cells, Skin Dendritic,Dendritic Cell, Dermal,Dendritic Cell, Epidermal,Dendritic Cell, Skin,Dermal Dendritic Cell,Epidermal Dendritic Cell,Skin Dendritic Cell
D008807 Mice, Inbred BALB C An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research. BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D002452 Cell Count The number of CELLS of a specific kind, usually measured per unit volume or area of sample. Cell Density,Cell Number,Cell Counts,Cell Densities,Cell Numbers,Count, Cell,Counts, Cell,Densities, Cell,Density, Cell,Number, Cell,Numbers, Cell
D004492 Edetic Acid A chelating agent that sequesters a variety of polyvalent cations such as CALCIUM. It is used in pharmaceutical manufacturing and as a food additive. EDTA,Edathamil,Edetates,Ethylenediaminetetraacetic Acid,Tetracemate,Calcium Disodium Edetate,Calcium Disodium Versenate,Calcium Tetacine,Chelaton 3,Chromium EDTA,Copper EDTA,Coprin,Dicobalt EDTA,Disodium Calcitetracemate,Disodium EDTA,Disodium Ethylene Dinitrilotetraacetate,Distannous EDTA,Edetate Disodium Calcium,Edetic Acid, Calcium Salt,Edetic Acid, Calcium, Sodium Salt,Edetic Acid, Chromium Salt,Edetic Acid, Dipotassium Salt,Edetic Acid, Disodium Salt,Edetic Acid, Disodium Salt, Dihydrate,Edetic Acid, Disodium, Magnesium Salt,Edetic Acid, Disodium, Monopotassium Salt,Edetic Acid, Magnesium Salt,Edetic Acid, Monopotassium Salt,Edetic Acid, Monosodium Salt,Edetic Acid, Potassium Salt,Edetic Acid, Sodium Salt,Ethylene Dinitrilotetraacetate,Ethylenedinitrilotetraacetic Acid,Gallium EDTA,Magnesium Disodium EDTA,N,N'-1,2-Ethanediylbis(N-(carboxymethyl)glycine),Potassium EDTA,Stannous EDTA,Versenate,Versene,Acid, Edetic,Acid, Ethylenediaminetetraacetic,Acid, Ethylenedinitrilotetraacetic,Calcitetracemate, Disodium,Dinitrilotetraacetate, Disodium Ethylene,Dinitrilotetraacetate, Ethylene,Disodium Versenate, Calcium,EDTA, Chromium,EDTA, Copper,EDTA, Dicobalt,EDTA, Disodium,EDTA, Distannous,EDTA, Gallium,EDTA, Magnesium Disodium,EDTA, Potassium,EDTA, Stannous,Edetate, Calcium Disodium,Ethylene Dinitrilotetraacetate, Disodium,Tetacine, Calcium,Versenate, Calcium Disodium
D004817 Epidermis The external, nonvascular layer of the skin. It is made up, from within outward, of five layers of EPITHELIUM: (1) basal layer (stratum basale epidermidis); (2) spinous layer (stratum spinosum epidermidis); (3) granular layer (stratum granulosum epidermidis); (4) clear layer (stratum lucidum epidermidis); and (5) horny layer (stratum corneum epidermidis).
D000375 Aging The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time. Senescence,Aging, Biological,Biological Aging
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001706 Biopsy Removal and pathologic examination of specimens from the living body. Biopsies
D013194 Staining and Labeling The marking of biological material with a dye or other reagent for the purpose of identifying and quantitating components of tissues, cells or their extracts. Histological Labeling,Staining,Histological Labelings,Labeling and Staining,Labeling, Histological,Labelings, Histological,Stainings
D013396 Suction The removal of secretions, gas or fluid from hollow or tubular organs or cavities by means of a tube and a device that acts on negative pressure. Aspiration, Mechanical,Drainage, Suction,Aspirations, Mechanical,Drainages, Suction,Mechanical Aspiration,Mechanical Aspirations,Suction Drainage,Suction Drainages,Suctions

Related Publications

S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
A convenient method to distinguish the epidermal from the dermal side in suction-blister epidermal grafting.
November 2013, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Superficial wounding model for epidermal barrier repair studies: comparison of Erbium:YAG laser and the suction blister method.
September 2012, Lasers in surgery and medicine,
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Suction blister epidermal grafting using a modified suction method in the treatment of stable vitiligo: a retrospective study.
July 2011, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
A comparative study of suction blister epidermal grafting and automated blister epidermal micrograft in stable vitiligo.
January 2022, Scientific reports,
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Pharmacokinetic studies of antibacterial agents using the suction blister method.
January 1990, Scandinavian journal of infectious diseases. Supplementum,
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Suction blister epidermal graft (SBEG) - an easy way to apply this method.
April 2019, Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG,
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Easy method to distinguish epidermal side through writing figure "4" over a suction blister.
September 2010, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
A simple way of performing suction blister epidermal grafting for melanocyte transplantation.
November 2014, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Suction blister epidermal grafting for management of eyelid vitiligo: special considerations.
March 2006, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.],
S T Langkjer, and J H Villadsen, and P Bjerring, and P Ebbesen
Identification of mononuclear cells in cell suspension from cutaneous infiltrates freed by a suction blister method.
January 1982, Acta dermato-venereologica,
Copied contents to your clipboard!