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The effect of gamma-radiation on terrilytin, a proteolytic enzyme immobilized on modified and nonmodified cellulose materials was studied by EPR. Dialdehyde cellulose and graft copolymer of cellulose and polyacrylic acid were used as the modified cellulose materials. Dependence of the native and immobilized terrilytin activity and the content of free radicals in the irradiated samples on the irradiation dose was observed. It was shown that immobilization of the enzyme led to increasing of its stability to the effect of the ionizing radiation. This was due to transfer of the free valency from terrilytin to the carrying polymer which prevented radiation and chemical destruction of the enzyme. The proteolytic activity of native terrilytin subjected to gamma-irradiation markedly decreased because of intramolecular and intermolecular interactions during reactions of the terrilytin free radicals, since in this case there was no polymer as an acceptor of the enzyme free valency.
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
May 1986,
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
January 1989,
Radiobiologiia,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
June 1986,
Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
January 1984,
Prikladnaia biokhimiia i mikrobiologiia,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
January 1984,
Farmakologiia i toksikologiia,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
October 1988,
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic],
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
January 1987,
Klinicheskaia khirurgiia,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
January 1974,
Meditsinskaia tekhnika,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
September 1972,
Problemy gematologii i perelivaniia krovi,
T N Iudanova, and
I F Skokova, and
E V Dovbiĭ, and
G A Maziĭ, and
A T Kalashnik
May 1965,
Biochimica et biophysica acta,
