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A sensitive and highly specific method for the determination of theophylline in serum by high-performance liquid chromatography (HPLC) has been developed. Theophylline was completely separated from paraxanthine, a major metabolite of caffeine which has been known to interfere with most isocratic reversed-phase HPLC methods, with a mixture of acetonitrile/tetrahydrofuran/acetate buffer (10 mM, pH 5.0; 5:1:94, v/v %) as the mobile phase using a C18 bonded reversed-phase column. Neither the other xanthine and uric acid derivatives nor numerous drugs tested were found to interfere. The proposed method was applied to therapeutic monitoring utilizing its excellent precision, reproducibility and high specificity for theophylline.
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
March 1980,
Clinical chemistry,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
September 1983,
Journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
July 1979,
Clinical chemistry,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
September 1997,
Se pu = Chinese journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
January 2002,
Se pu = Chinese journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
May 2000,
Se pu = Chinese journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
May 1998,
Se pu = Chinese journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
April 1995,
Journal of chromatography. B, Biomedical applications,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
December 1987,
Journal of chromatography,
K Kawakatsu, and
K Nishimura, and
M Kawai, and
M Chikuma
May 2002,
Se pu = Chinese journal of chromatography,
