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Antisense RNA was used to specifically inhibit the expression of a GUS gene introduced in a transgenic plant. A tobacco transformant containing a single intact copy of the GUS gene and showing relatively high constitutive levels of GUS activity (GUS +) was re-transformed with an Agrobacterium Ti-derived binary vector containing an antisense version of this reporter gene. The sense and antisense GUS genes were each under the regulation of the CaMV 35S promoter. Re-transformed plants contained 1-5 copies of the antisense construct and all showed a greater than 90% reduction in GUS activity relative to the original GUS + plant. This reduction in GUS activity correlated closely with the levels of GUS enzyme and steady state GUS mRNA observed in these plants. The relatively low levels of sense and antisense GUS transcripts found in the re-transformed plants may indicate a rapid degradation of the RNA:RNA duplex in the cell.
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
November 1994,
Biological chemistry Hoppe-Seyler,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
May 1988,
Plant molecular biology,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
July 1990,
Plant molecular biology,
L S Robert, and
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I Altosaar, and
P G Arnison, and
S F Fabijanski
October 1993,
Plant molecular biology,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
August 1990,
Molekuliarnaia genetika, mikrobiologiia i virusologiia,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
October 1990,
Plant molecular biology,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
September 1994,
Transgenic research,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
December 1989,
Proceedings of the National Academy of Sciences of the United States of America,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
March 1988,
Molecular & general genetics : MGG,
L S Robert, and
P A Donaldson, and
C Ladaique, and
I Altosaar, and
P G Arnison, and
S F Fabijanski
September 1989,
Proceedings of the National Academy of Sciences of the United States of America,
