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Both the phosphatidylinositol-hydrolysing and the phosphatidylcholine-hydrolysing phospholipases C have been implicated in the generation of second messengers in mammalian cells. The phosphatidylcholine-hydrolysing phospholipase C (PLC) from Bacillus cereus, a monomeric protein containing 245 amino-acid residues, is similar to some of the corresponding mammalian proteins. This, together with the fact that the bacterial enzyme can mimic the action of mammalian PLC in causing, for example, enhanced prostaglandin biosynthesis, suggests that B. cereus PLC can be used as a model for the hitherto poorly characterized mammalian PLCs. We report here the three-dimensional structure of B. cereus PLC at 1.5 A resolution. The enzyme is an all-helix protein belonging to a novel structural class and contains, at least in the crystalline state, three Zn2+ in the active site. We also present preliminary results from a study at 1.9 A resolution of the complex between PLC and inorganic phosphate (Pi) which indicate that the substrate binds directly to the metal ions.
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
December 1969,
Biochimica et biophysica acta,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
August 1995,
The EMBO journal,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
March 2007,
Applied microbiology and biotechnology,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
September 2007,
Proteins,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
October 1977,
European journal of biochemistry,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
December 1965,
Biochimica et biophysica acta,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
June 1999,
Journal of biochemistry,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
February 1982,
Scandinavian journal of clinical and laboratory investigation,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
August 1995,
Analytical biochemistry,
E Hough, and
L K Hansen, and
B Birknes, and
K Jynge, and
S Hansen, and
A Hordvik, and
C Little, and
E Dodson, and
Z Derewenda
March 1993,
Biophysical journal,
