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The effects of recombinant human interferon (IFN) alpha-2b and gamma on the bone marrow megakaryocyte progenitors (CFU-Meg) were compared between eight patients in the chronic phase of Ph1-positive chronic myelocytic leukemia (CML) and five hematologically normal patients. CFU-Meg was assayed in plasma clot culture added with phytohemagglutinin-stimulated leukocyte-conditioned medium as a source of colony stimulating activity. The average count of CFU-Meg colonies formed from the bone marrow of CML patients was 5.5 times that of normal controls. Spontaneous CFU-Meg colonies were grown in seven of eight CML patients, but in none of five controls. Colony formation by CFU-Meg in CML as well as normal bone marrow was suppressed by the two preparations of IFN in a dose dependent fashion. Their suppressive influence on colonies from CFU-Meg was comparable between CML and normal bone marrow at lower concentrations, but was less marked for CML than normal bone marrow at higher concentrations. The formation of CFU-Meg colonies from CML bone marrow was more severely suppressed by IFN-gamma than IFN-alpha-2b. Depletion of either T lymphocytes or adherent cells from the CML bone marrow cells diminished the suppressive effects of IFN-gamma, but had no influence on the effects of IFN-alpha-2b.
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
August 1984,
Nihon Ketsueki Gakkai zasshi : journal of Japan Haematological Society,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
May 1987,
Experimental hematology,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
October 1987,
Blood,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
October 1990,
Experimental hematology,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
July 1987,
Experimental hematology,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
February 2001,
Human & experimental toxicology,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
April 1987,
Japanese journal of pharmacology,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
February 1989,
International journal of cancer,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
October 1987,
Blood,
Y Tanabe, and
K Dan, and
S Kuriya, and
T Nomura
December 1984,
Laboratory investigation; a journal of technical methods and pathology,
