-transparent.png){kind=logo}
Interferons (IFNs) have been shown to suppress the proliferation of human pluripotent hematopoietic progenitor cells, CFU-GEMM, and committed erythroid (BFU-E, CFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells. However, no information is yet available concerning the effect of IFNs on human megakaryocytic progenitor cells CFU-Mk. Furthermore the mechanisms underlying the inhibitory activity of IFNs are still controversial. Therefore highly purified recombinant IFN preparations, rIFN-alpha and rIFN-gamma, were assessed for their influence on in vitro growth of human bone marrow-derived CFU-Mk as well as CFU-GEMM. In addition, the role of hematopoietic accessory cells, that is, adherent cells and T lymphocytes, in the mediation of the suppressive effect of rIFNs was examined. When added to unseparated bone marrow cells, both rIFN preparations significantly inhibited colony formation with 50% inhibition of CFU-Mk occurring at 22 U/mL for rIFN-alpha and 59 U/mL for rIFN-gamma, while 50% inhibition of CFU-GEMM occurred at 59 U/mL for rIFN-alpha and 101 U/mL for rIFN-gamma. The suppressive effect of rIFN-alpha and rIFN-gamma was selectively abolished by monoclonal antibodies (MoAbs) against rIFN-alpha and rIFN-gamma, thus confirming that the inhibitory activity was due to the rIFN preparations used. The antiproliferative effect of rIFN-alpha and rIFN-gamma on CFU-GEMM growth was not associated with a decrease in the percentage of mixed colonies containing megakaryocytic cells as assessed by use of the MoAb C17.28 against platelet glycoprotein IIIa. Removal of adherent cells and T lymphocytes from the target bone marrow cells had no influence on the suppressive effect of rIFN-alpha, whereas it significantly reduced the inhibitory effect of rIFN-gamma on the growth of megakaryocytic colonies and the other hematopoietic progenitors. The data indicate that (1) human megakaryocytopoiesis is markedly inhibited by rIFN-alpha and rIFN-gamma, and (2) the inhibitory effect of rIFN-alpha is due to a direct action on hematopoietic progenitor cells, whereas the effect of rIFN-gamma is mediated to a significant degree through accessory cell populations.
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
April 1992,
AIDS research and human retroviruses,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
September 1989,
Blood,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
October 2004,
Experimental cell research,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
December 1990,
The Keio journal of medicine,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
January 1993,
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
April 1987,
Journal of biological response modifiers,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
December 1984,
Laboratory investigation; a journal of technical methods and pathology,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
October 1989,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
August 1996,
Blood,
A Ganser, and
C Carlo-Stella, and
J Greher, and
B Völkers, and
D Hoelzer
January 2000,
Methods in molecular biology (Clifton, N.J.),
