-transparent.png){kind=logo}
A previous paper reported application of L-asparaginase IgG from Vibrio succinogenes in screening for positive clones. This paper describes the detailed procedure for preparation of high-purity IgG, an essential step of which involves coupling of a cell-free extract of host E. coli Y 1090 to Sepharose 4B beads and adsorption of the non-specific antigen components by passage of the polyvalent IgG through this affinity column. The IgG thus obtained exhibited improved specificity and was utilized as a radioimmunologic and horseradish peroxidase-linked immunologic probe in screening for positive clones. These IgG probes had the advantages of low background, high sensitivity, and good reliability.
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
January 1979,
Preparative biochemistry,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
January 1974,
Applied microbiology,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
July 1985,
Journal of molecular biology,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
November 1976,
The Journal of biological chemistry,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
October 1979,
Applied and environmental microbiology,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
November 2003,
Nippon Ganka Gakkai zasshi,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
September 2016,
Molecular biotechnology,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
July 1978,
Applied and environmental microbiology,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
October 1996,
European journal of biochemistry,
Y Q Guan, and
H L Pong, and
Y S He, and
J M Chen, and
K Q Ren
January 1992,
[Rinsho ketsueki] The Japanese journal of clinical hematology,
