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A highly enriched population of bovine T lymphocytes was produced from peripheral blood leukocytes following the depletion of monoclonal antibody-labelled B lymphocytes and monocytes with magnetic microspheres. This negative-enrichment protocol was simple, rapid, and specific. Also, it had a high recovery efficiency and was consistently reproducible. The enriched T lymphocytes proliferated in response to recombinant bovine interleukin 2 and, following the addition of monocytes, to concanavalin A. This methodology made it possible to determine the proliferative responses of peripheral blood lymphocytes utilizing a constant number of T lymphocytes within each assay. In this way, the in vitro T lymphocyte responses were determined independent of changes in the number of responder cells within peripheral blood.
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May 1985,
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P J Griebel, and
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P J Griebel, and
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P J Griebel, and
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April 1982,
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P J Griebel, and
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P J Griebel, and
A P Gee, and
L Qualtiere, and
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P J Griebel, and
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P J Griebel, and
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L A Babiuk
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P J Griebel, and
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