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A new technique for depletion of T cells from bone marrow is presented. Bone marrow cells (BMC) were rosetted with magnetic monosized polystyrene microspheres coated with monoclonal antibodies (MAbs) specific for T cell CD2 and CD3 antigens. Rosetted T cells were subsequently removed from non-T cells with the aid of a magnet. This immunomagnetic separation procedure was carried out in less than 40 min and reproducibly removed T cells, leaving a maximum of 0.025% sheep-red-blood-cell (SRBC) rosette-forming cells and less than 0.02% T cells as detected by a T cell limiting dilution assay. The efficacy of the depletion procedure was further shown by flow cytometry data, by effective removal of cells from a T cell line added to the BMC prior to immunomagnetic separation, and by abrogation of interleukin 2 (IL-2)-producing capacity in T-cell-depleted BMC (BMC-T). The T cell depletion procedure provided a 43-74% recovery of non-T cells present in the Isopaque-Ficoll-isolated bone marrow mononuclear cell fraction and did not disturb the growth potential of stem cells, as assayed by hematopoietic stem cell assays.
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G Kvalheim, and
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G Gaudernack, and
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D Albrechtsen
August 1987,
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F Vartdal, and
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D Albrechtsen
May 1989,
Scandinavian journal of immunology,
F Vartdal, and
G Kvalheim, and
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G Gaudernack, and
J Ugelstad, and
D Albrechtsen
January 1985,
Progress in clinical and biological research,
F Vartdal, and
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V Bosnes, and
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D Albrechtsen
February 1982,
British journal of haematology,
F Vartdal, and
G Kvalheim, and
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V Bosnes, and
G Gaudernack, and
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D Albrechtsen
October 1987,
Zhonghua yi xue za zhi = Chinese medical journal; Free China ed,
F Vartdal, and
G Kvalheim, and
T E Lea, and
V Bosnes, and
G Gaudernack, and
J Ugelstad, and
D Albrechtsen
January 1985,
Progress in clinical and biological research,
F Vartdal, and
G Kvalheim, and
T E Lea, and
V Bosnes, and
G Gaudernack, and
J Ugelstad, and
D Albrechtsen
August 1987,
Bone marrow transplantation,
F Vartdal, and
G Kvalheim, and
T E Lea, and
V Bosnes, and
G Gaudernack, and
J Ugelstad, and
D Albrechtsen
November 1986,
British journal of cancer,
F Vartdal, and
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J Ugelstad, and
D Albrechtsen
September 1989,
Veterinary immunology and immunopathology,
F Vartdal, and
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V Bosnes, and
G Gaudernack, and
J Ugelstad, and
D Albrechtsen
January 1984,
Lancet (London, England),
