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Techniques for analyzing DNA distributions on agarose gels are examined by both two-dimensional and one-dimensional methods. It is demonstrated that very large errors in DNA concentration occur in such analyses unless (i) the electrophoresis is performed in a careful, reproducible manner, (ii) the films are calibrated with an internal standard, (iii) high resolution densitometry is used for analyzing the films, and (iv) appropriate background controls are used to determine the baselines for integration. Two-dimensional scanning produces more accurate results than one-dimensional scanning, but in cases where the bands are relatively uniform, the one-dimensional analysis gives good results. A technique for determining accurate distributions is described.
E A Ribeiro, and
L L Larcom, and
D P Miller
June 1987,
Analytical biochemistry,
E A Ribeiro, and
L L Larcom, and
D P Miller
June 1982,
Journal of biochemical and biophysical methods,
E A Ribeiro, and
L L Larcom, and
D P Miller
January 1993,
Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society,
E A Ribeiro, and
L L Larcom, and
D P Miller
June 1994,
Biophysical chemistry,
E A Ribeiro, and
L L Larcom, and
D P Miller
November 1997,
BioTechniques,
E A Ribeiro, and
L L Larcom, and
D P Miller
December 1996,
Biochemistry,
E A Ribeiro, and
L L Larcom, and
D P Miller
April 1994,
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
E A Ribeiro, and
L L Larcom, and
D P Miller
January 2000,
Acta biochimica Polonica,
E A Ribeiro, and
L L Larcom, and
D P Miller
February 1997,
Journal of biochemical and biophysical methods,
E A Ribeiro, and
L L Larcom, and
D P Miller
June 1984,
Analytical biochemistry,
