Biomaterial Database

Differential intracellular staining of identified neurones in Locusta with texas red and lucifer yellow. 1989

H Schneider

Universität Konstanz, Fakultät für Biologie, F.R.G.

The bright red fluorescent dye, Texas red, is introduced for ionophoretic microinjection in conjunction with the well-known dye Lucifer yellow. Different identified neurones can thus be visualised in two strongly contrasting colours in the same preparation (differential intracellular staining) following their physiological characterisation. Satisfactory results were obtained with electrodes filled with 4% Texas red (sulforhodamine 101 acid chloride; w/v) in 1 M potassium acetate (pH 3.0) and 5% Lucifer yellow (w/v) in aqua dest., respectively. Texas red was injected ionophoretically with pulsed depolarising current (3-10 nA, 500 ms pulses at 1 Hz, 15-30 min) and Lucifer yellow with hyperpolarizing constant current (5-6 nA, 5-15 min). Histological tissue processing was identical for both dyes, the quality of intracellular recordings with Texas red electrodes was similar to that with Lucifer yellow electrodes. Stained neurones could be visualised in both whole-mounts and sectioned preparations. Differential staining of two identified synaptically coupled neurones, a motoneurone and an interneurone, in the mesothorax of Locusta is presented as an illustration for the possible localisation of contact sites at the light-microscopic level.

UI	MeSH Term	Description	Entries
D007395	Interneurons	Most generally any NEURONS which are not motor or sensory. Interneurons may also refer to neurons whose AXONS remain within a particular brain region in contrast to projection neurons, which have axons projecting to other brain regions.	Intercalated Neurons,Intercalated Neuron,Interneuron,Neuron, Intercalated,Neurons, Intercalated
D007546	Isoquinolines	A group of compounds with the heterocyclic ring structure of benzo(c)pyridine. The ring structure is characteristic of the group of opium alkaloids such as papaverine. (From Stedman, 25th ed)
D008297	Male		Males
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D005260	Female		Females
D005456	Fluorescent Dyes	Chemicals that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.	Flourescent Agent,Fluorescent Dye,Fluorescent Probe,Fluorescent Probes,Fluorochrome,Fluorochromes,Fluorogenic Substrates,Fluorescence Agents,Fluorescent Agents,Fluorogenic Substrate,Agents, Fluorescence,Agents, Fluorescent,Dyes, Fluorescent,Probes, Fluorescent,Substrates, Fluorogenic
D006110	Grasshoppers	Plant-eating orthopterans having hindlegs adapted for jumping. There are two main families: Acrididae and Romaleidae. Some of the more common genera are: Melanoplus, the most common grasshopper; Conocephalus, the eastern meadow grasshopper; and Pterophylla, the true katydid.	Acrididae,Locusts,Romaleidae,Grasshopper,Locust
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D014966	Xanthenes	Compounds with three aromatic rings in linear arrangement with an OXYGEN in the center ring.	Xanthene