-transparent.png){kind=logo}
A new approach to gene mapping which combines enzymatic amplification with high-resolution flow sorting of human chromosomes has been devised. Reliable amplification from as few as 200 chromosomes has been demonstrated. This method, with particular application to mapping the position of chromosomal translocations, has been used to show that the breakpoint for the constitutional translocation t(11;22)(q23;q11) lies proximal to the genes c-ets-1, Thy-1, and T3 delta and distal to the int-2 gene. The mapping was confirmed by Southern analysis to much larger numbers of chromosomes sorted from the same cell line. Control reactions for the bcl-2 gene on chromosome 18 and the C alpha gene of the IGH locus on chromosome 14 demonstrated the discrimination which can be achieved.
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 1989,
Nucleic acids research,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 1987,
Methods in enzymology,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
November 2003,
BioTechniques,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
February 1992,
Genomics,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
June 2008,
BMC genomics,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 2016,
Methods in molecular biology (Clifton, N.J.),
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 2014,
Genes & genetic systems,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
May 1994,
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 1979,
Chromosoma,
F Cotter, and
S Nasipuri, and
G Lam, and
B D Young
January 1990,
Cytometry,
