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Acoustophoretic removal of proteins from blood components. 2015

Maria Tenje, and Maria N Lundgren, and Ann-Margret Swärd-Nilsson, and Jens Kjeldsen-Kragh, and Lena Lyxe, and Andreas Lenshof
Department of Biomedical Engineering, Lund University, Lund, Sweden, maria.tenje@angstrom.uu.se.

This work presents the development of a miniaturized system for removing plasma proteins and other low-molecular-weight compounds from red blood cell (RBC) concentrate in a simple one-step-process using integrated ultrasound. The technology utilizes the principles of acoustophoresis to transfer the RBCs from the original plasma-containing solution into a protein-free SAG-M additive solution in a continuous flow process. The preparation of protein free RBC concentrate is important for blood transfusion to patients suffering from immunoglobulin A (IgA)-deficiency and developing antibodies against IgA. We show a nearly complete removal of both albumin and IgA from concentrated RBCs via this one-step-processes in samples obtained from RBC concentrate. The cell recovery of our technology is close to 97%, compared to just above 90% of the current procedure of repeated dilution and centrifugation steps. This work clearly shows the potential of integrated acoustophoresis in a miniaturized system for clinical applications.

UI MeSH Term Description Entries
D008904 Miniaturization The design or construction of objects greatly reduced in scale. Miniaturisation,Miniaturisations,Miniaturizations
D001781 Blood Component Removal Any procedure in which blood is withdrawn from a donor, a portion is separated and retained, at the same time the remainder is returned to the donor. Apheresis,Pheresis,Aphereses,Blood Component Removals,Phereses,Removal, Blood Component
D001798 Blood Proteins Proteins that are present in blood serum, including SERUM ALBUMIN; BLOOD COAGULATION FACTORS; and many other types of proteins. Blood Protein,Plasma Protein,Plasma Proteins,Serum Protein,Serum Proteins,Protein, Blood,Protein, Plasma,Protein, Serum,Proteins, Blood,Proteins, Plasma,Proteins, Serum
D004586 Electrophoresis An electrochemical process in which macromolecules or colloidal particles with a net electric charge migrate in a solution under the influence of an electric current. Electrophoreses
D004867 Equipment Design Methods and patterns of fabricating machines and related hardware. Design, Equipment,Device Design,Medical Device Design,Design, Medical Device,Designs, Medical Device,Device Design, Medical,Device Designs, Medical,Medical Device Designs,Design, Device,Designs, Device,Designs, Equipment,Device Designs,Equipment Designs
D004912 Erythrocytes Red blood cells. Mature erythrocytes are non-nucleated, biconcave disks containing HEMOGLOBIN whose function is to transport OXYGEN. Blood Cells, Red,Blood Corpuscles, Red,Red Blood Cells,Red Blood Corpuscles,Blood Cell, Red,Blood Corpuscle, Red,Erythrocyte,Red Blood Cell,Red Blood Corpuscle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000162 Acoustics The branch of physics that deals with sound and sound waves. In medicine it is often applied in procedures in speech and hearing studies. With regard to the environment, it refers to the characteristics of a room, auditorium, theatre, building, etc. that determines the audibility or fidelity of sounds in it. (From Random House Unabridged Dictionary, 2d ed) Acoustic
D013010 Sonication The application of high intensity ultrasound to liquids. Sonications
D056656 Lab-On-A-Chip Devices Microdevices that combine microfluidics technology with electrical and/or mechanical functions for analyzing very small fluid volumes. They consist of microchannels etched into substrates made of silicon, glass, or polymer using processes similar to photolithography. The test fluids in the channels can then interact with different elements such as electrodes, photodetectors, chemical sensors, pumps, and valves. Microchip Analytical Devices,Microfluidic Devices,Microfluidic Lab-On-A-Chip,Microfluidic Microchips,Nanochip Analytical Devices,Analytical Device, Microchip,Analytical Device, Nanochip,Analytical Devices, Microchip,Analytical Devices, Nanochip,Device, Lab-On-A-Chip,Device, Microchip Analytical,Device, Microfluidic,Device, Nanochip Analytical,Devices, Lab-On-A-Chip,Devices, Microchip Analytical,Devices, Microfluidic,Devices, Nanochip Analytical,Lab On A Chip Devices,Lab-On-A-Chip Device,Lab-On-A-Chip, Microfluidic,Lab-On-A-Chips, Microfluidic,Microchip Analytical Device,Microchip, Microfluidic,Microchips, Microfluidic,Microfluidic Device,Microfluidic Lab On A Chip,Microfluidic Lab-On-A-Chips,Microfluidic Microchip,Nanochip Analytical Device

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