Development of a solid-phase competition ELISA to detect antibodies against newly emerged Duck Tembusu virus. 2015

Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, PR China.

Duck Tembusu virus (DTMUV) was first isolated in Northern China in 2010, and is an emerging viral pathogen that is now rapidly spreading to most Chinese provinces causing a severe disease and economic losses for the duck breeding industry. The virus can affect ducks, chickens and geese, but currently there are no commercial ELISA methods for detecting antibodies against DTMUV from different species, or for assessing antibody titers in infected birds. In the present study, a solid-phase competition ELISA for this purpose was developed. The sensitivity and specificity of this test were 97.6% and 100%, respectively, when the percent inhibition (PI) was set at 15% using a serum dilution of 1:16. No cross-reaction with the sera against other avian viral pathogens was found in the study and the ELISA results generated for duck and chicken sera from the field were totally consistent with those of the classical virus neutralization assay. Therefore, this solid-phase competition ELISA represents a sensitive, specific and quantitative tool for the serological detection of Tembusu virus in different birds to facilitate the prevention and control of this disease.

UI MeSH Term Description Entries
D011201 Poultry Diseases Diseases of birds which are raised as a source of meat or eggs for human consumption and are usually found in barnyards, hatcheries, etc. The concept is differentiated from BIRD DISEASES which is for diseases of birds not considered poultry and usually found in zoos, parks, and the wild. Disease, Poultry,Diseases, Poultry,Poultry Disease
D002645 Chickens Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA. Gallus gallus,Gallus domesticus,Gallus gallus domesticus,Chicken
D002681 China A country spanning from central Asia to the Pacific Ocean. Inner Mongolia,Manchuria,People's Republic of China,Sinkiang,Mainland China
D004372 Ducks A water bird in the order Anseriformes (subfamily Anatinae (true ducks)) with a broad blunt bill, short legs, webbed feet, and a waddling gait. Duck
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D005416 Flavivirus A genus of FLAVIVIRIDAE containing several subgroups and many species. Most are arboviruses transmitted by mosquitoes or ticks. The type species is YELLOW FEVER VIRUS. Arbovirus, Group B,Arboviruses, Group B,Flaviviruses,Group B Arbovirus,Group B Arboviruses
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000914 Antibodies, Viral Immunoglobulins produced in response to VIRAL ANTIGENS. Viral Antibodies
D012680 Sensitivity and Specificity Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed) Specificity,Sensitivity,Specificity and Sensitivity
D018177 Flavivirus Infections Infections with viruses of the genus FLAVIVIRUS, family FLAVIVIRIDAE. Infections, Flavivirus,Flavivirus Infection,Infection, Flavivirus

Related Publications

Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
January 2012, PloS one,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
May 2024, Journal of virological methods,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
March 2015, Archives of virology,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
November 2017, Journal of virological methods,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
February 2016, Monoclonal antibodies in immunodiagnosis and immunotherapy,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
May 2020, Journal of virological methods,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
October 2023, Poultry science,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
October 2011, Virology journal,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
June 2019, Avian diseases,
Yuguang Fu, and Yanhong Ji, and Bin Liu, and Rihab Muhammad Dafallah, and Qiyun Zhu
January 2018, Frontiers in microbiology,
Copied contents to your clipboard!