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Monoclonal antibody based solid phase competition ELISA to detect FMDV serotype A specific antibodies. 2024
In Foot-and-Mouth disease (FMD) enzootic countries, periodic vaccination is the key tool in controlling the disease incidence. Active seromonitoring of vaccinated population is critical to assess the impact of vaccination. Virus neutralization test (VNT) and enzyme-linked immunosorbent assays (ELISA) are commonly used for antibody detection. Assays like liquid phase blocking ELISA (LPBE) or solid phase competition ELISA (SPCE) are preferred as they do not require handling of FMDV and are are routinely used for seromonitoring or vaccine potency testing; however, false positives are high in LPBE. Here we report, a monoclonal antibody (mAb) based SPCE as a potential alternate assay for antibody titration. From a panel of 12 mAbs against FMDV serotype A, two mAbs were chosen for the development of SPCE. Based on a panel of 453 sera, it was demonstrated the mAb 2C4G11, mAb 6E8D11and pAb based SPCE had a relative sensitivity of 86.1, 86.1 and 80.3%; and specificity of 99.6, 99.1 and 99.1%, respectively. The correlation, repeatability, and level of agreement of the aforementioned assays were high demonstrating the potential use of mAb in large scale surveillance studies and regular vaccine potency testing.
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