Cocaine-induced locomotor sensitization in rats correlates with nucleus accumbens activity on manganese-enhanced MRI. 2015

Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
Department of Psychiatry and Behavioral Neurosciences, Wayne State University School of Medicine, Detroit, MI, USA.

A long-standing goal of substance abuse research has been to link drug-induced behavioral outcomes with the activity of specific brain regions to understand the neurobiology of addiction behaviors and to search for drug-able targets. Here, we tested the hypothesis that cocaine produces locomotor (behavioral) sensitization that correlates with increased calcium channel-mediated neuroactivity in brain regions linked with drug addiction, such as the nucleus accumbens (NAC), anterior striatum (AST) and hippocampus, as measured using manganese-enhanced MRI (MEMRI). Rats were treated with cocaine for 5 days, followed by a 2-day drug-free period. The following day, locomotor sensitization was quantified as a metric of cocaine-induced neuroplasticity in the presence of manganese. Immediately following behavioral testing, rats were examined for changes in calcium channel-mediated neuronal activity in the NAC, AST, hippocampus and temporalis muscle, which was associated with behavioral sensitization using MEMRI. Cocaine significantly increased locomotor activity and produced behavioral sensitization compared with saline treatment of control rats. A significant increase in MEMRI signal intensity was determined in the NAC, but not AST or hippocampus, of cocaine-treated rats compared with saline-treated control rats. Cocaine did not increase signal intensity in the temporalis muscle. Notably, in support of our hypothesis, behavior was significantly and positively correlated with MEMRI signal intensity in the NAC. As neuronal uptake of manganese is regulated by calcium channels, these results indicate that MEMRI is a powerful research tool to study neuronal activity in freely behaving animals and to guide new calcium channel-based therapies for the treatment of cocaine abuse and dependence.

UI MeSH Term Description Entries
D008124 Locomotion Movement or the ability to move from one place or another. It can refer to humans, vertebrate or invertebrate animals, and microorganisms. Locomotor Activity,Activities, Locomotor,Activity, Locomotor,Locomotor Activities
D008279 Magnetic Resonance Imaging Non-invasive method of demonstrating internal anatomy based on the principle that atomic nuclei in a strong magnetic field absorb pulses of radiofrequency energy and emit them as radiowaves which can be reconstructed into computerized images. The concept includes proton spin tomographic techniques. Chemical Shift Imaging,MR Tomography,MRI Scans,MRI, Functional,Magnetic Resonance Image,Magnetic Resonance Imaging, Functional,Magnetization Transfer Contrast Imaging,NMR Imaging,NMR Tomography,Tomography, NMR,Tomography, Proton Spin,fMRI,Functional Magnetic Resonance Imaging,Imaging, Chemical Shift,Proton Spin Tomography,Spin Echo Imaging,Steady-State Free Precession MRI,Tomography, MR,Zeugmatography,Chemical Shift Imagings,Echo Imaging, Spin,Echo Imagings, Spin,Functional MRI,Functional MRIs,Image, Magnetic Resonance,Imaging, Magnetic Resonance,Imaging, NMR,Imaging, Spin Echo,Imagings, Chemical Shift,Imagings, Spin Echo,MRI Scan,MRIs, Functional,Magnetic Resonance Images,Resonance Image, Magnetic,Scan, MRI,Scans, MRI,Shift Imaging, Chemical,Shift Imagings, Chemical,Spin Echo Imagings,Steady State Free Precession MRI
D008297 Male Males
D008345 Manganese A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
D009473 Neuronal Plasticity The capacity of the NERVOUS SYSTEM to change its reactivity as the result of successive activations. Brain Plasticity,Plasticity, Neuronal,Axon Pruning,Axonal Pruning,Dendrite Arborization,Dendrite Pruning,Dendritic Arborization,Dendritic Pruning,Dendritic Remodeling,Neural Plasticity,Neurite Pruning,Neuronal Arborization,Neuronal Network Remodeling,Neuronal Pruning,Neuronal Remodeling,Neuroplasticity,Synaptic Plasticity,Synaptic Pruning,Arborization, Dendrite,Arborization, Dendritic,Arborization, Neuronal,Arborizations, Dendrite,Arborizations, Dendritic,Arborizations, Neuronal,Axon Prunings,Axonal Prunings,Brain Plasticities,Dendrite Arborizations,Dendrite Prunings,Dendritic Arborizations,Dendritic Prunings,Dendritic Remodelings,Network Remodeling, Neuronal,Network Remodelings, Neuronal,Neural Plasticities,Neurite Prunings,Neuronal Arborizations,Neuronal Network Remodelings,Neuronal Plasticities,Neuronal Prunings,Neuronal Remodelings,Neuroplasticities,Plasticities, Brain,Plasticities, Neural,Plasticities, Neuronal,Plasticities, Synaptic,Plasticity, Brain,Plasticity, Neural,Plasticity, Synaptic,Pruning, Axon,Pruning, Axonal,Pruning, Dendrite,Pruning, Dendritic,Pruning, Neurite,Pruning, Neuronal,Pruning, Synaptic,Prunings, Axon,Prunings, Axonal,Prunings, Dendrite,Prunings, Dendritic,Prunings, Neurite,Prunings, Neuronal,Prunings, Synaptic,Remodeling, Dendritic,Remodeling, Neuronal,Remodeling, Neuronal Network,Remodelings, Dendritic,Remodelings, Neuronal,Remodelings, Neuronal Network,Synaptic Plasticities,Synaptic Prunings
D009714 Nucleus Accumbens Collection of pleomorphic cells in the caudal part of the anterior horn of the LATERAL VENTRICLE, in the region of the OLFACTORY TUBERCLE, lying between the head of the CAUDATE NUCLEUS and the ANTERIOR PERFORATED SUBSTANCE. It is part of the so-called VENTRAL STRIATUM, a composite structure considered part of the BASAL GANGLIA. Accumbens Nucleus,Nucleus Accumbens Septi,Accumbens Septi, Nucleus,Accumbens Septus, Nucleus,Accumbens, Nucleus,Nucleus Accumbens Septus,Nucleus, Accumbens,Septi, Nucleus Accumbens,Septus, Nucleus Accumbens
D003042 Cocaine An alkaloid ester extracted from the leaves of plants including coca. It is a local anesthetic and vasoconstrictor and is clinically used for that purpose, particularly in the eye, ear, nose, and throat. It also has powerful central nervous system effects similar to the amphetamines and is a drug of abuse. Cocaine, like amphetamines, acts by multiple mechanisms on brain catecholaminergic neurons; the mechanism of its reinforcing effects is thought to involve inhibition of dopamine uptake. Cocaine HCl,Cocaine Hydrochloride,HCl, Cocaine,Hydrochloride, Cocaine
D003287 Contrast Media Substances used to allow enhanced visualization of tissues. Radiopaque Media,Contrast Agent,Contrast Agents,Contrast Material,Contrast Materials,Radiocontrast Agent,Radiocontrast Agents,Radiocontrast Media,Agent, Contrast,Agent, Radiocontrast,Agents, Contrast,Agents, Radiocontrast,Material, Contrast,Materials, Contrast,Media, Contrast,Media, Radiocontrast,Media, Radiopaque
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
February 2020, Behavioural brain research,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
January 2008, The European journal of neuroscience,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
April 2003, The Journal of pharmacology and experimental therapeutics,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
June 2000, Pharmacology, biochemistry, and behavior,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
April 2002, Behavioural brain research,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
July 1998, Pharmacology, biochemistry, and behavior,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
July 2013, Behavioural brain research,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
January 2018, Frontiers in behavioral neuroscience,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
May 1996, Neuroreport,
Shane A Perrine, and Farhad Ghoddoussi, and Kirtan Desai, and Robert J Kohler, and Ajay T Eapen, and Michael J Lisieski, and Mariana Angoa-Perez, and Donald M Kuhn, and Kelly E Bosse, and Alana C Conti, and David Bissig, and Bruce A Berkowitz
November 2009, The European journal of neuroscience,
Copied contents to your clipboard!