Biomaterial Database

Evaluation of two rapid methods to screen pathogens from stool specimens. 1989

T A Geers, and B A Backes

Catherine McAuley Health Center, St. Joseph Mercy Hospital, Ann Arbor, Michigan 48106.

Two rapid methods, the Enteric Pathogen Screen (EPS) cards of the AutoMicrobic system (Vitek Systems, Inc., Hazelwood, MO) and the Bactigen Salmonella-Shigella latex agglutination (LPA) method (Wampole Laboratories, Cranbury, NJ) were compared with conventional biochemical tests to screen stool cultures having suspicious colonial morphologic characteristics for the presence of Salmonella and Shigella in a study of 481 isolates from stool specimens and 104 stock cultures. Compared with conventional testing, overall, 327 of 394 (83%) clinically irrelevant organisms resulted in a report of no Salmonella spp., Shigella spp., or Yersinia enterocolitica with EPS. Four hundred forty-nine of 457 (98%) of clinically irrelevant organisms yielded negative LPA results. Because the LPA method is faster, eliminates more clinically irrelevant organisms from further testing, and does not require the use of an expensive identification system, the authors believe that it is better suited for direct screening for Salmonella and Shigella for most clinical laboratories.

UI	MeSH Term	Description	Entries
D007841	Latex Fixation Tests	Passive agglutination tests in which antigen is adsorbed onto latex particles which then clump in the presence of antibody specific for the adsorbed antigen. (From Stedman, 26th ed)	Latex Agglutination Tests,Agglutination Test, Latex,Agglutination Tests, Latex,Fixation Test, Latex,Fixation Tests, Latex,Latex Agglutination Test,Latex Fixation Test,Test, Latex Agglutination,Test, Latex Fixation,Tests, Latex Agglutination,Tests, Latex Fixation
D005188	False Negative Reactions	Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)	False Negative Reaction,Reaction, False Negative,Reactions, False Negative
D005189	False Positive Reactions	Positive test results in subjects who do not possess the attribute for which the test is conducted. The labeling of healthy persons as diseased when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)	False Positive Reaction,Positive Reaction, False,Positive Reactions, False,Reaction, False Positive,Reactions, False Positive
D005243	Feces	Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D012475	Salmonella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that utilizes citrate as a sole carbon source. It is pathogenic for humans, causing enteric fevers, gastroenteritis, and bacteremia. Food poisoning is the most common clinical manifestation. Organisms within this genus are separated on the basis of antigenic characteristics, sugar fermentation patterns, and bacteriophage susceptibility.
D012760	Shigella	A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that ferments sugar without gas production. Its organisms are intestinal pathogens of man and other primates and cause bacillary dysentery (DYSENTERY, BACILLARY).
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor