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We have obtained sequence data from five proteolytic peptides totalling 102 amino acid residues of the 80 kDa protein kinase C substrate purified from rat brain. The amino acid sequences of these five peptides were compared with that deduced from a cDNA encoding the 87 kDa protein kinase C substrate from bovine brain. The overall amino acid sequence identity within the regions covered by our peptides is 54%. Two peptides aligned at the C- and N-termini of the bovine protein kinase C substrate with a very high degree of homology (more than 80% identity). Two other peptides exhibited 62% and 46% identity with two regions located in the C-terminal half of the bovine protein. The fifth peptide which contains the sequence PEQPEQPEQ did not reveal any similarity with the bovine protein. Based on the homologies of our experimentally determined sequences, which represent about 30% of the deduced sequence of the bovine protein, we suggest that although these protein kinase C substrates are not identical, they may belong to a family of related proteins.
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
July 1990,
FEBS letters,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
October 1987,
Proceedings of the National Academy of Sciences of the United States of America,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
May 1990,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
November 1990,
The Biochemical journal,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
July 1987,
European journal of biochemistry,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
June 1989,
Proceedings of the National Academy of Sciences of the United States of America,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
October 1992,
Chemical & pharmaceutical bulletin,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
September 1993,
Archives of biochemistry and biophysics,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
April 1988,
FEBS letters,
J D Erusalimsky, and
C Morris, and
K Perks, and
R Brown, and
S Brooks, and
E Rozengurt
November 1990,
European journal of biochemistry,
