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Dimethyl sulfoxide is a widely accepted and recommended solvent in which to dissolve compounds to be tested for mutagenicity via the Ames Salmonella/mammalian microsome assay. Using tester strains TA98 and TA100, we observed a bacteriotoxic response with various fractions isolated from beer when dissolved in DMSO but not when dissolved in water. Further characterization of the role of solvent in simple model systems consisting of butanol, DMSO and bacteria strongly suggests a chemical reaction occurs between dimethyl sulfoxide and specific chemical constituents of the test substance, nutrient broth, or the Ames bacterial strains. The result of such an interaction could be misinterpreted as a toxic response to the test substance when, in fact, the bacteriotoxicity could be due to another compound, chemically distinct from the test substance.
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
November 1993,
Mutagenesis,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
December 2016,
Mutation research. Genetic toxicology and environmental mutagenesis,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
November 1993,
Mutation research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
August 1981,
Mutation research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
January 1981,
Mutation research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
January 1998,
SAR and QSAR in environmental research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
February 1998,
Zentralblatt fur Hygiene und Umweltmedizin = International journal of hygiene and environmental medicine,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
June 1995,
Mutation research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
February 1980,
Mutation research,
D J Vaughan, and
J A Baptista, and
G R Perdomo, and
J J Krepinsky
September 1998,
Mutation research,
