BIOMAT Database Logo BIOMAT Database Logo

Polymer Based Gene Silencing: In Vitro Delivery of SiRNA. 2016

Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
UCL School of Pharmacy, 29-39 Brunswick Square, London, WC1N 1AX, UK.

Gene silencing may be achieved by harnessing the RNA interference mechanism to effect down-regulation of protein expression. The therapeutic use of siRNA is dependent on its delivery to the intracellular space. This chapter describes the delivery of siRNA by N-(2-ethylamino)-6-O-glycolchitosan (EAGC). EAGC is a chitosan-based polymer, which binds to siRNA to form nanoparticles (NPs). The steps necessary to determine the delivery capacity of a polymer are presented in this chapter using EAGC as an example. The steps include: the transfection of cells with EAGC-siRNA polyplexes and protein detection by a Western Blotting assay.

UI MeSH Term Description Entries
D008081 Liposomes Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins. Niosomes,Transferosomes,Ultradeformable Liposomes,Liposomes, Ultra-deformable,Liposome,Liposome, Ultra-deformable,Liposome, Ultradeformable,Liposomes, Ultra deformable,Liposomes, Ultradeformable,Niosome,Transferosome,Ultra-deformable Liposome,Ultra-deformable Liposomes,Ultradeformable Liposome
D010316 Particle Size Relating to the size of solids. Particle Sizes,Size, Particle,Sizes, Particle
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D045744 Cell Line, Tumor A cell line derived from cultured tumor cells. Tumor Cell Line,Cell Lines, Tumor,Line, Tumor Cell,Lines, Tumor Cell,Tumor Cell Lines
D048271 Chitosan Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS. Poliglusam
D053758 Nanoparticles Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging. Nanocrystalline Materials,Nanocrystals,Material, Nanocrystalline,Materials, Nanocrystalline,Nanocrystal,Nanocrystalline Material,Nanoparticle
D020868 Gene Silencing Interruption or suppression of the expression of a gene at transcriptional or translational levels. Gene Inactivation,Inactivation, Gene,Silencing, Gene
D034741 RNA, Small Interfering Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions. RNA, Scan,Repeat-Associated siRNA,Scan RNA,Small Scan RNA,Trans-Acting siRNA,siRNA,siRNA, Repeat-Associated,siRNA, Trans-Acting,Short Hairpin RNA,Short Interfering RNA,Small Hairpin RNA,Small Interfering RNA,scnRNA,shRNA,tasiRNA,Hairpin RNA, Short,Hairpin RNA, Small,Interfering RNA, Short,Interfering RNA, Small,RNA, Short Hairpin,RNA, Short Interfering,RNA, Small Hairpin,RNA, Small Scan,Repeat Associated siRNA,Scan RNA, Small,Trans Acting siRNA,siRNA, Repeat Associated,siRNA, Trans Acting

Related Publications

Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Hybrid lipid-polymer nanoparticles for sustained siRNA delivery and gene silencing.
July 2014, Nanomedicine : nanotechnology, biology, and medicine,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Cyclodextrin-containing polymer delivery system for light-directed siRNA gene silencing.
August 2010, Oligonucleotides,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
DNA tetrahedron-based nanogels for siRNA delivery and gene silencing.
April 2019, Chemical communications (Cambridge, England),
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Delivery strategies for siRNA-mediated gene silencing.
April 2006, Current drug delivery,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system.
November 2010, Biomaterials,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing.
October 2017, Biomaterials science,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Polymeric nanoparticles for siRNA delivery and gene silencing.
February 2009, International journal of pharmaceutics,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Modified cyclodextrin-based nanoparticles mediated delivery of siRNA for huntingtin gene silencing across an in vitro BBB model.
December 2021, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Surface Engineered Dendrimers in siRNA Delivery and Gene Silencing.
January 2017, Current pharmaceutical design,
Margarida I Simão Carlos, and Andreas Schätzlein, and Ijeoma Uchegbu
Nonviral siRNA delivery for gene silencing in neurodegenerative diseases.
January 2010, Methods in molecular biology (Clifton, N.J.),
Copied contents to your clipboard!