Defined pH-sensitive nanogels as gene delivery platform for siRNA mediated in vitro gene silencing. 2017

Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
Institute of Chemistry and Biochemistry, Freie Universität Berlin, Takustrasse 3, Berlin 14195, Germany. haag@chemie.fu-berlin.de nan.ma@hzg.de.

In the present study, a pH sensitive nanogel platform for gene delivery was developed. The cationic nanogels based on dendritic polyglycerol (dPG) and low molecular weight polyethylenimine units were able to encapsulate siRNA during the manufacturing process. The thiol-Michael nanoprecipitation method, which operates under mild conditions and did not require any catalyst or surfactant, was used to develop tailor-made nanogels in the sub-100 nm range. The incorporation of pH sensitive benzacetal-bonds inside the nanogel network enables the controlled intracellular release of the cargo. The functionality to transport therapeutic biomolecules was tested by an in vitro GFP-siRNA transfection assay. Encapsulated siRNA could silence GFP expressing HeLa cells (up to 71% silencing in GFP). Furthermore, significantly reduced toxicity of the nanogel platform compared to the non-degradable PEI was observed. These properties realize a new carrier platform in the field of gene therapy.

UI MeSH Term Description Entries
D011092 Polyethylene Glycols Polymers of ETHYLENE OXIDE and water, and their ethers. They vary in consistency from liquid to solid depending on the molecular weight indicated by a number following the name. They are used as SURFACTANTS, dispersing agents, solvents, ointment and suppository bases, vehicles, and tablet excipients. Some specific groups are NONOXYNOLS, OCTOXYNOLS, and POLOXAMERS. Macrogols,Polyoxyethylenes,Carbowax,Macrogol,Polyethylene Glycol,Polyethylene Oxide,Polyethyleneoxide,Polyglycol,Glycol, Polyethylene,Glycols, Polyethylene,Oxide, Polyethylene,Oxides, Polyethylene,Polyethylene Oxides,Polyethyleneoxides,Polyglycols,Polyoxyethylene
D011094 Polyethyleneimine Strongly cationic polymer that binds to certain proteins; used as a marker in immunology, to precipitate and purify enzymes and lipids. Synonyms: aziridine polymer; Epamine; Epomine; ethylenimine polymer; Montrek; PEI; Polymin(e). Polyaziridine,Polyethylenimine,Polyaziridines,Polyethyleneimines,Polyethylenimines
D004337 Drug Carriers Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers. Drug Carrier
D005782 Gels Colloids with a solid continuous phase and liquid as the dispersed phase; gels may be unstable when, due to temperature or other cause, the solid phase liquefies; the resulting colloid is called a sol.
D006367 HeLa Cells The first continuously cultured human malignant CELL LINE, derived from the cervical carcinoma of Henrietta Lacks. These cells are used for, among other things, VIRUS CULTIVATION and PRECLINICAL DRUG EVALUATION assays. Cell, HeLa,Cells, HeLa,HeLa Cell
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D014162 Transfection The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES. Transfections
D053758 Nanoparticles Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging. Nanocrystalline Materials,Nanocrystals,Material, Nanocrystalline,Materials, Nanocrystalline,Nanocrystal,Nanocrystalline Material,Nanoparticle
D020868 Gene Silencing Interruption or suppression of the expression of a gene at transcriptional or translational levels. Gene Inactivation,Inactivation, Gene,Silencing, Gene

Related Publications

Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
April 2019, Chemical communications (Cambridge, England),
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
April 2006, Current drug delivery,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
August 2013, Molecular pharmaceutics,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
November 2012, Journal of controlled release : official journal of the Controlled Release Society,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
January 2022, EXCLI journal,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
January 2016, Methods in molecular biology (Clifton, N.J.),
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
October 2002, Nature biotechnology,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
August 2019, Nanoscale research letters,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
February 2021, Biomaterials science,
Mathias Dimde, and Falko Neumann, and Felix Reisbeck, and Svenja Ehrmann, and Jose Luis Cuellar-Camacho, and Dirk Steinhilber, and Nan Ma, and Rainer Haag
July 2012, Accounts of chemical research,
Copied contents to your clipboard!