Urea Transporter B and MicroRNA-200c Differ in Kidney Outer Versus Inner Medulla Following Dehydration. 2016

Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
Department of Medicine, Renal Division, Emory University School of Medicine, Atlanta, GA; Tongji Hospital, Tongji University, Shanghai, China.

Urea transporters (UTs) are important in urine concentration and in urea recycling, and UT-B has been implicated in both. In kidney, UT-B was originally localized to outer medullary descending vasa recta, and more recently detected in inner medullary descending vasa recta. Endogenously produced microRNAs (miRs) bind to the 3'UTR of genes and generally inhibit their translation, thus playing a pivotal role gene regulation. Mice were dehydrated for 24 hours then sacrificed. Inner and outer medullas were analyzed by polymerase chain reaction (PCR) and quantitative PCR for miRNA expression and analyzed by western blotting for protein abundance. MiRNA sequencing analysis of mouse inner medullas showed a 40% increase in miRNA-200c in dehydrated mice compared with controls. An in silico analysis of the targets for miR-200c revealed that miRNA-200c could directly target the gene for UT-B. PCR confirmed that miR-200c is up-regulated in the inner medullas of dehydrated mice while western blot showed that UT-B protein abundance was down-regulated in the same portion of the kidney. However, in the outer medulla, miR-200c was reduced and UT-B protein was increased in dehydrated mice. This is the first indication that UT-B protein and miR-200c may each be differentially regulated by dehydration within the kidney outer and inner medulla. The inverse correlation between the direction of change in miR-200c and UT-B protein abundance in both the inner and outer medulla suggests that miR-200c may be associated with the change in UT-B protein in these 2 portions of the kidney medulla.

UI MeSH Term Description Entries
D007679 Kidney Medulla The internal portion of the kidney, consisting of striated conical masses, the renal pyramids, whose bases are adjacent to the cortex and whose apices form prominent papillae projecting into the lumen of the minor calyces. Kidney Papilla,Kidney Medullas,Kidney Papillas,Medulla, Kidney,Medullas, Kidney,Papilla, Kidney,Papillas, Kidney
D008297 Male Males
D008810 Mice, Inbred C57BL One of the first INBRED MOUSE STRAINS to be sequenced. This strain is commonly used as genetic background for transgenic mouse models. Refractory to many tumors, this strain is also preferred model for studying role of genetic variations in development of diseases. Mice, C57BL,Mouse, C57BL,Mouse, Inbred C57BL,C57BL Mice,C57BL Mice, Inbred,C57BL Mouse,C57BL Mouse, Inbred,Inbred C57BL Mice,Inbred C57BL Mouse
D003681 Dehydration The condition that results from excessive loss of water from a living organism. Water Stress,Stress, Water
D005786 Gene Expression Regulation Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation. Gene Action Regulation,Regulation of Gene Expression,Expression Regulation, Gene,Regulation, Gene Action,Regulation, Gene Expression
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D015536 Down-Regulation A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Down-Regulation,Down-Regulation (Physiology),Downregulation,Down Regulation,Down-Regulation, Receptor
D015854 Up-Regulation A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins. Receptor Up-Regulation,Upregulation,Up-Regulation (Physiology),Up Regulation
D060888 Real-Time Polymerase Chain Reaction Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction. Kinetic Polymerase Chain Reaction,Quantitative Real-Time PCR,Quantitative Real-Time Polymerase Chain Reaction,Real-Time PCR,PCR, Quantitative Real-Time,PCR, Real-Time,PCRs, Quantitative Real-Time,PCRs, Real-Time,Quantitative Real Time PCR,Quantitative Real Time Polymerase Chain Reaction,Quantitative Real-Time PCRs,Real Time PCR,Real Time Polymerase Chain Reaction,Real-Time PCR, Quantitative,Real-Time PCRs,Real-Time PCRs, Quantitative

Related Publications

Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
December 2003, American journal of physiology. Regulatory, integrative and comparative physiology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
August 2005, Molecular and cellular biology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
December 1997, The American journal of physiology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
December 2015, American journal of physiology. Renal physiology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
November 1991, The American journal of physiology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
January 1997, Biochimica et biophysica acta,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
October 1988, Health visitor,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
May 1986, The American journal of otology,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
January 1980, The International journal of biochemistry,
Juan Wang, and Xiaonan H Wang, and Haidong Wang, and Ling Chen, and Janet D Klein, and Jeff M Sands
March 2001, American journal of physiology. Renal physiology,
Copied contents to your clipboard!