Biomaterial Database

Phorbol-12-myristate-13-acetate (PMA) mediated transcriptional regulation of Oncostatin-M. 2016

Srimoyee Mukherjee, and Sumita Sengupta Bandyopadhyay

Department of Biophysics, Molecular Biology and Bioinformatics, University of Calcutta, 92 A.P.C. Road, Kolkata 700009, India.

Oncostatin-M (OSM), an IL-6 family cytokine, exhibits varied roles in different patho-physiological conditions. Differential expression of OSM in response to varying stimuli indicates importance of its regulation of expression. The present study illustrated transcriptional induction of osm on treatment with chemical inducer, phorbol-12-myristate-13-acetate (PMA). Following initial hours of PMA treatment, a nuclear protein C/EBP-β binds specifically to the CCAAT consensus sequence at the proximal end of the OSM promoter. Genistein (a specific Tyr phosphorylation inhibitor) leads to the interaction of CHOP (C/EBP Homologous Protein) with C/EBP-β, thus negatively regulating it. Knockdown of C/EBP-β also leads to inhibition of PMA-mediated OSM induction.

UI	MeSH Term	Description	Entries
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D013755	Tetradecanoylphorbol Acetate	A phorbol ester found in CROTON OIL with very effective tumor promoting activity. It stimulates the synthesis of both DNA and RNA.	Phorbol Myristate Acetate,12-Myristoyl-13-acetylphorbol,12-O-Tetradecanoyl Phorbol 13-Acetate,Tetradecanoylphorbol Acetate, 4a alpha-Isomer,12 Myristoyl 13 acetylphorbol,12 O Tetradecanoyl Phorbol 13 Acetate,13-Acetate, 12-O-Tetradecanoyl Phorbol,Acetate, Phorbol Myristate,Acetate, Tetradecanoylphorbol,Myristate Acetate, Phorbol,Phorbol 13-Acetate, 12-O-Tetradecanoyl,Tetradecanoylphorbol Acetate, 4a alpha Isomer
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription
D051742	Transcription Factor CHOP	A CCAAT-enhancer binding protein that is induced by DNA DAMAGE and growth arrest. It serves as a dominant negative inhibitor of other CCAAT-enhancer binding proteins.	C-EBP Homologous Protein,DDIT3 Protein,DNA Damage-Inducible Transcript 3 Protein,GADD153 Protein,Growth Arrest And DNA Damage-Inducible Protein 153,C EBP Homologous Protein,CHOP, Transcription Factor,DNA Damage Inducible Transcript 3 Protein,Growth Arrest And DNA Damage Inducible Protein 153,Homologous Protein, C-EBP
D053683	Oncostatin M	A cytokine with both pro- and anti-inflammatory actions that depend upon the cellular microenvironment. Oncostatin M is a 28 kDa monomeric glycoprotein that is similar in structure to LEUKEMIA INHIBITORY FACTOR. Its name derives from the the observation that it inhibited the growth of tumor cells and augmented the growth of normal fibroblasts.
D019833	Genistein	An isoflavonoid derived from soy products. It inhibits PROTEIN-TYROSINE KINASE and topoisomerase-II (DNA TOPOISOMERASES, TYPE II); activity and is used as an antineoplastic and antitumor agent. Experimentally, it has been shown to induce G2 PHASE arrest in human and murine cell lines and inhibits PROTEIN-TYROSINE KINASE.	Genestein
D020218	Response Elements	Nucleotide sequences, usually upstream, which are recognized by specific regulatory transcription factors, thereby causing gene response to various regulatory agents. These elements may be found in both promoter and enhancer regions.	Element, Response,Elements, Response,Response Element
D020298	U937 Cells	A human cell line established from a diffuse histiocytic lymphoma (HISTIOCYTIC LYMPHOMA, DIFFUSE) and displaying many monocytic characteristics. It serves as an in vitro model for MONOCYTE and MACROPHAGE differentiation.	Cell, U937,Cells, U937,U937 Cell
D022782	CCAAT-Enhancer-Binding Protein-beta	A CCAAT-enhancer-binding protein found in LIVER; INTESTINES; LUNG and ADIPOSE TISSUE. It is an important mediator of INTERLEUKIN-6 signaling.	C-EBP-beta,NF-IL6,23-C-EBP Protein,40-C-EBP Protein,AGP-EBP Transcription Factor,C-EBP beta,C-EBP-Related Protein 2,C-EBPbeta,CRP2 Protein,IL-6 DBP,IL-6-Dependent DNA Binding Protein,Interleukin-6 Nuclear Factor,LAP Transcription Factor,Liver-Enriched Inhibiting Protein,Liver-Enriched Inhibitory Protein, LIP,2, C-EBP-Related Protein,AGP EBP Transcription Factor,C EBP Related Protein 2,C EBP beta,C EBPbeta,CCAAT Enhancer Binding Protein beta,IL 6 Dependent DNA Binding Protein,Inhibiting Protein, Liver-Enriched,Interleukin 6 Nuclear Factor,Liver Enriched Inhibiting Protein,Liver Enriched Inhibitory Protein, LIP,Nuclear Factor, Interleukin-6,Protein-beta, CCAAT-Enhancer-Binding,Transcription Factor, AGP-EBP,Transcription Factor, LAP