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We successfully detected the oxyradical production in human synovial A (macrophage-like) and B (fibroblast-like) cells by phorbol 12-myristate 13-acetate (PMA) using the luminol-chemiluminescence method. The PMA (0.1 microgram/ml)-induced photon generation was abolished by an O2- scavenger, superoxide dismutase, and an H2O2 scavenger, catalase, suggesting that the stimulus produced oxyradicals in synovial cells. Both of these responses were abolished by a protein kinase C (PKC) inhibitor, calphostine C, but unaffected by an intracellular Ca2+ chelator, BAPTA-AM, and Ca2+ removal from the extracellular medium. These findings suggest that synovial A and B cells produce oxyradicals through PKC-mediated and [Ca2+]i-independent mechanisms, probably through the activation of NADPH oxidase.
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
December 2016,
Cytokine,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
September 2007,
Biochemical pharmacology,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
August 1991,
The Biochemical journal,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
January 2015,
BioMed research international,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
September 2019,
The journal of physiological sciences : JPS,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
November 1994,
Biochemical pharmacology,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
May 2009,
Archives of biochemistry and biophysics,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
January 2019,
Proteomics insights,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
February 2010,
Molecular and cellular biochemistry,
T Tanabe, and
H Otani, and
K Mishima, and
R Ogawa, and
C Inagaki
July 1980,
Cancer letters,
